Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3666057..3666736 Replicon chromosome
Accession NZ_CP031293
Organism Escherichia coli strain EC17GD31

Toxin (Protein)


Gene name higB Uniprot ID A0A0A1A1R1
Locus tag DWB25_RS17815 Protein ID WP_000057541.1
Coordinates 3666434..3666736 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag DWB25_RS17810 Protein ID WP_000806442.1
Coordinates 3666057..3666398 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DWB25_RS17800 3662300..3663232 - 933 WP_000883039.1 glutaminase A -
DWB25_RS17805 3663495..3665999 + 2505 WP_061092784.1 copper-exporting P-type ATPase CopA -
DWB25_RS17810 3666057..3666398 - 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
DWB25_RS17815 3666434..3666736 - 303 WP_000057541.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DWB25_RS17820 3666869..3667663 + 795 WP_000365157.1 TraB/GumN family protein -
DWB25_RS17825 3667867..3668346 + 480 WP_000186626.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
DWB25_RS17830 3668383..3670035 - 1653 WP_000771723.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
DWB25_RS17835 3670253..3671473 + 1221 WP_001251634.1 fosmidomycin MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11795.37 Da        Isoelectric Point: 10.2638

>T108855 WP_000057541.1 NZ_CP031293:c3666736-3666434 [Escherichia coli]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T108855 NZ_CP031293:c3666736-3666434 [Escherichia coli]
ATGGCTCAAAAGAATAATATAAGGAGTTTTCGTGATGCCTGGCTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTGTCCGGGAAATTACAGGAATATTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATATTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT108855 WP_000806442.1 NZ_CP031293:c3666398-3666057 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT108855 NZ_CP031293:c3666398-3666057 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTACTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAATAATCGTAAACTCACCACTG
AAATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A1A1R1

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References