Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2399247..2399809 Replicon chromosome
Accession NZ_CP031293
Organism Escherichia coli strain EC17GD31

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag DWB25_RS11405 Protein ID WP_000605676.1
Coordinates 2399531..2399809 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag DWB25_RS11400 Protein ID WP_000781370.1
Coordinates 2399247..2399531 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DWB25_RS11385 2394606..2397653 + 3048 WP_012311994.1 formate dehydrogenase-N subunit alpha -
DWB25_RS11390 2397666..2398550 + 885 WP_001240582.1 formate dehydrogenase N subunit beta -
DWB25_RS11395 2398543..2399196 + 654 WP_000045650.1 formate dehydrogenase-N subunit gamma -
DWB25_RS11400 2399247..2399531 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
DWB25_RS11405 2399531..2399809 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DWB25_RS11410 2399995..2401005 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
DWB25_RS11415 2401139..2402836 - 1698 WP_000433464.1 malate dehydrogenase -
DWB25_RS11420 2402993..2403130 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
DWB25_RS11425 2403232..2403447 - 216 WP_000495766.1 biofilm-dependent modulation protein -
DWB25_RS11430 2403792..2404223 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T108845 WP_000605676.1 NZ_CP031293:c2399809-2399531 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T108845 NZ_CP031293:c2399809-2399531 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGCTGTCTTGCAGATATCGATATGCCCGGTTACCGATTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGGATAACGTTCGAGTTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT108845 WP_000781370.1 NZ_CP031293:c2399531-2399247 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT108845 NZ_CP031293:c2399531-2399247 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCTATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGCCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References