Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3070388..3070950 Replicon chromosome
Accession NZ_CP031256
Organism Escherichia coli strain M16807

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag DV447_RS15435 Protein ID WP_000605675.1
Coordinates 3070388..3070666 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag DV447_RS15440 Protein ID WP_000781370.1
Coordinates 3070666..3070950 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DV447_RS15405 3065974..3066405 - 432 WP_000152307.1 peroxiredoxin OsmC -
DV447_RS15415 3066750..3066965 + 216 WP_000495766.1 biofilm-dependent modulation protein -
DV447_RS15420 3067067..3067204 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
DV447_RS15425 3067361..3069058 + 1698 WP_000433460.1 malate dehydrogenase -
DV447_RS15430 3069192..3070202 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
DV447_RS15435 3070388..3070666 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DV447_RS15440 3070666..3070950 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
DV447_RS15445 3071179..3071832 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
DV447_RS15450 3071825..3072709 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
DV447_RS15455 3072722..3075769 - 3048 WP_011076399.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T108757 WP_000605675.1 NZ_CP031256:3070388-3070666 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T108757 NZ_CP031256:3070388-3070666 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGTAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT108757 WP_000781370.1 NZ_CP031256:3070666-3070950 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT108757 NZ_CP031256:3070666-3070950 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCTACGGCAAGTCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTGTCTGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References