108745

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1688415..1689142 Replicon chromosome
Accession NZ_CP031256
Organism Escherichia coli strain M16807

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag DV447_RS08500 Protein ID WP_000547555.1
Coordinates 1688415..1688726 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H2V9Z2
Locus tag DV447_RS08505 Protein ID WP_000126293.1
Coordinates 1688723..1689142 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DV447_RS08470 1683556..1685265 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
DV447_RS08475 1685275..1685817 + 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
DV447_RS08480 1685817..1686584 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
DV447_RS08485 1686581..1686991 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
DV447_RS08490 1686984..1687454 + 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
DV447_RS08495 1687497..1688252 + 756 WP_001305268.1 hypothetical protein -
DV447_RS08500 1688415..1688726 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
DV447_RS08505 1688723..1689142 + 420 WP_000126293.1 helix-turn-helix domain-containing protein Antitoxin
DV447_RS08510 1689234..1689662 - 429 WP_000536064.1 hypothetical protein -
DV447_RS08520 1690047..1690574 + 528 WP_001078777.1 electron transport protein HydN -
DV447_RS08525 1690727..1692979 + 2253 WP_001107659.1 carbamoyltransferase HypF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T108745 WP_000547555.1 NZ_CP031256:1688415-1688726 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T108745 NZ_CP031256:1688415-1688726 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCTTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATCACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15426.46 Da        Isoelectric Point: 4.4596

>AT108745 WP_000126293.1 NZ_CP031256:1688723-1689142 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT108745 NZ_CP031256:1688723-1689142 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCAGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATACCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2V9Z2

References