Detailed information of TA system TA108740

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 257254..257856
• Replicon: chromosome
• Accession: NZ_CP031256
• Organism: Escherichia coli strain M16807

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: DV447_RS01230
• Protein ID: WP_000897302.1
• Coordinates: 257545..257856 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DV447_RS01225
• Protein ID: WP_000356397.1
• Coordinates: 257254..257544 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DV447_RS01190	253327..254229	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
DV447_RS01195	254226..254861	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
DV447_RS01200	254858..255787	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
DV447_RS01205	256003..256221	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
DV447_RS01215	256617..256895	-	279	WP_001296612.1	hypothetical protein	-
DV447_RS01225	257254..257544	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
DV447_RS01230	257545..257856	-	312	WP_000897302.1	hypothetical protein	Toxin
DV447_RS01235	258086..258994	+	909	WP_001305059.1	alpha/beta hydrolase	-
DV447_RS01240	259058..259999	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
DV447_RS01245	260044..260481	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
DV447_RS01250	260478..261350	-	873	WP_000920762.1	virulence factor BrkB family protein	-
DV447_RS01255	261344..261943	-	600	WP_001443191.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T108740 WP_000897302.1 NZ_CP031256:c257856-257545 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T108740 NZ_CP031256:c257856-257545 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT108740 WP_000356397.1 NZ_CP031256:c257544-257254 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT108740 NZ_CP031256:c257544-257254 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416