Detailed information of TA system TA108736

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1489710..1490379
• Replicon: chromosome
• Accession: NZ_CP031254
• Organism: Haemophilus influenzae strain M25588

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2R3G0F4
• Locus tag: DV401_RS07540
• Protein ID: WP_015701504.1
• Coordinates: 1489710..1490093 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A502K5Z3
• Locus tag: DV401_RS07545
• Protein ID: WP_005651224.1
• Coordinates: 1490077..1490379 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DV401_RS07505	1486470..1486793	+	324	WP_114877442.1	DUF2190 family protein	-
DV401_RS07510	1486774..1487085	+	312	WP_015701509.1	hypothetical protein	-
DV401_RS07515	1487088..1487618	+	531	WP_015701508.1	phage tail protein	-
DV401_RS07520	1487627..1488037	+	411	WP_005651206.1	phage tail protein	-
DV401_RS07525	1488040..1488690	+	651	WP_114877444.1	hypothetical protein	-
DV401_RS07530	1488965..1489372	+	408	WP_114877446.1	hypothetical protein	-
DV401_RS07535	1489417..1489641	+	225	WP_005651221.1	DUF4035 domain-containing protein	-
DV401_RS07540	1489710..1490093	+	384	WP_015701504.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DV401_RS07545	1490077..1490379	+	303	WP_005651224.1	XRE family transcriptional regulator	Antitoxin
DV401_RS07550	1490395..1490631	+	237	WP_101494098.1	hypothetical protein	-
DV401_RS07555	1490683..1492611	+	1929	WP_114877448.1	tape measure protein	-
DV401_RS07560	1492581..1492955	+	375	WP_114877450.1	phage minor tail protein L	-
DV401_RS07565	1492958..1493707	+	750	WP_112102023.1	C40 family peptidase	-
DV401_RS07570	1493644..1494258	+	615	WP_112072211.1	tail assembly protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	kdsA	1454718..1519634	64916

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-110)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-81)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14868.05 Da
• Isoelectric Point: 4.4987

>T108736 WP_015701504.1 NZ_CP031254:1489710-1490093 [Haemophilus influenzae]
MKQEWEVILQDPLLNWLETLAEDDVLKIYAALELLSTEGPQLSRPYADTLQGSKYTNLKELRVQSKLSVFRLFYIFDPVR
QAIVLCGGDKKGKKEKLFYKEMIALAEQTYDDYLSELTKEQENEREI

Nucleotide

• Length: 384 bp

>T108736 NZ_CP031254:1489710-1490093 [Haemophilus influenzae]
ATGAAACAAGAATGGGAAGTAATTTTACAAGACCCACTTTTGAACTGGTTAGAAACGCTGGCAGAAGATGACGTATTAAA
AATCTATGCGGCGTTGGAATTATTATCAACAGAAGGTCCACAATTAAGCAGACCTTATGCGGATACGCTGCAAGGCTCTA
AATATACCAATTTAAAAGAATTGCGAGTACAGTCTAAATTATCGGTATTCCGTTTATTTTATATTTTCGACCCTGTCAGA
CAAGCGATTGTTTTATGTGGCGGAGATAAAAAAGGCAAGAAAGAAAAACTCTTTTACAAAGAGATGATTGCCCTAGCGGA
ACAAACCTATGATGATTACCTTTCTGAATTAACTAAGGAGCAAGAAAATGAGCGTGAAATTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11258.21 Da
• Isoelectric Point: 9.5655

>AT108736 WP_005651224.1 NZ_CP031254:1490077-1490379 [Haemophilus influenzae]
MSVKFKDLMNNLPAEKQAKVKAMADDMRMELQLYRIREELELSQKQMAEALSISQPSVVALEKRGNDIKLSSVKRYIEAM
GGVLNLSVELPTGKTVTFNL

Nucleotide

• Length: 303 bp

>AT108736 NZ_CP031254:1490077-1490379 [Haemophilus influenzae]
ATGAGCGTGAAATTTAAAGATCTGATGAATAATCTTCCAGCTGAAAAGCAAGCGAAAGTGAAAGCTATGGCAGACGATAT
GCGAATGGAGTTACAACTTTACCGTATTCGTGAAGAATTAGAGCTTTCACAAAAGCAAATGGCAGAAGCATTAAGTATTT
CTCAGCCGTCAGTTGTCGCCCTTGAAAAACGTGGCAATGACATTAAATTATCATCAGTTAAACGTTACATTGAAGCAATG
GGTGGTGTGTTAAATTTATCGGTTGAATTACCCACAGGAAAAACAGTTACTTTCAACTTATAG

Structures

Toxin

• AlphaFold DB: A0A2R3G0F4

Antitoxin

• AlphaFold DB: A0A502K5Z3