Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 993097..993736 Replicon chromosome
Accession NZ_CP031243
Organism Haemophilus haemolyticus strain M19346

Toxin (Protein)


Gene name higB Uniprot ID A0A1B8PGE6
Locus tag DV428_RS04970 Protein ID WP_012054677.1
Coordinates 993097..993402 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID H1LMA4
Locus tag DV428_RS04975 Protein ID WP_009499989.1
Coordinates 993413..993736 (+) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DV428_RS04945 989135..990271 + 1137 WP_114908887.1 DUF2827 family protein -
DV428_RS04950 990316..990375 - 60 WP_114909578.1 hypothetical protein -
DV428_RS04955 990435..991087 + 653 Protein_934 IS1595 family transposase -
DV428_RS04960 991173..992147 - 975 WP_114908888.1 zinc transporter permease subunit ZevB -
DV428_RS04965 992150..992770 - 621 WP_114908889.1 zinc transporter binding subunit ZevA -
DV428_RS04970 993097..993402 + 306 WP_012054677.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DV428_RS04975 993413..993736 + 324 WP_009499989.1 HigA family addiction module antidote protein Antitoxin
DV428_RS04980 993901..995571 + 1671 WP_114908890.1 energy-dependent translational throttle protein EttA -
DV428_RS04985 995633..995974 + 342 WP_005634743.1 SirB2 family protein -
DV428_RS04990 995979..997949 + 1971 WP_114908892.1 tRNA(Met) cytidine acetyltransferase TmcA -
DV428_RS09615 997946..998119 + 174 WP_162790776.1 DUF5363 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 990435..990983 548


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(7-97)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-67)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 12180.80 Da        Isoelectric Point: 8.3426

>T108705 WP_012054677.1 NZ_CP031243:993097-993402 [Haemophilus haemolyticus]
MFNLKREHFRDDYLYRFYQYGDAHSKIPSNLSKVLARKLDMISAAEDINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Download         Length: 306 bp

>T108705 NZ_CP031243:993097-993402 [Haemophilus haemolyticus]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATGCTCATAGTAAGAT
TCCTTCTAATTTATCTAAAGTATTGGCAAGAAAATTGGATATGATAAGTGCTGCAGAAGACATCAATGATTTACGTTCTC
CGCCGGCTAATCACTTAGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCCATTCGAGTAAATAAACAATATCGCCTA
ATTTTTAAATATGAGAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTATAA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 12302.14 Da        Isoelectric Point: 7.4050

>AT108705 WP_009499989.1 NZ_CP031243:993413-993736 [Haemophilus haemolyticus]
MMTRKPTSVGEILQEEFLEPLSLKINDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKAFDTTPEFWLNLQTRIDLWEL
EHNKRFQQSLANVKPALHRHGTSTFAM

Download         Length: 324 bp

>AT108705 NZ_CP031243:993413-993736 [Haemophilus haemolyticus]
ATGATGACGAGAAAACCCACTTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAATGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAACATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGCATTTGATACCACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGAGCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGGTACTTCTACATTTGCAAT
GTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B8PGE6

Antitoxin

Source ID Structure
AlphaFold DB H1LMA4

References