Detailed information of TA system TA108698

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 128952..129591
• Replicon: chromosome
• Accession: NZ_CP031242
• Organism: Haemophilus influenzae strain M21460

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: DV390_RS00560
• Protein ID: WP_005650215.1
• Coordinates: 128952..129257 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DV390_RS00565
• Protein ID: WP_005650217.1
• Coordinates: 129268..129591 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DV390_RS00540	124382..126421	-	2040	WP_042594251.1	excinuclease ABC subunit B	-
DV390_RS00550	127036..128004	-	969	WP_005666730.1	zinc transporter permease subunit ZevB	-
DV390_RS00555	128007..128627	-	621	WP_042594250.1	zinc transporter binding subunit ZevA	-
DV390_RS00560	128952..129257	+	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DV390_RS00565	129268..129591	+	324	WP_005650217.1	HigA family addiction module antidote protein	Antitoxin
DV390_RS00570	129754..131424	+	1671	WP_005650218.1	energy-dependent translational throttle protein EttA	-
DV390_RS00575	131486..131827	+	342	WP_005652996.1	SirB2 family protein	-
DV390_RS00580	131832..133802	+	1971	WP_011961904.1	tRNA(Met) cytidine acetyltransferase TmcA	-
DV390_RS09280	133799..133972	+	174	WP_011272700.1	DUF5363 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T108698 WP_005650215.1 NZ_CP031242:128952-129257 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T108698 NZ_CP031242:128952-129257 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCTATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT108698 WP_005650217.1 NZ_CP031242:129268-129591 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT108698 NZ_CP031242:129268-129591 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70