Detailed information of TA system TA108694

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 594659..595298
• Replicon: chromosome
• Accession: NZ_CP031241
• Organism: Haemophilus influenzae strain M17648

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: DV384_RS02935
• Protein ID: WP_005650215.1
• Coordinates: 594993..595298 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DV384_RS02930
• Protein ID: WP_005650217.1
• Coordinates: 594659..594982 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DV384_RS09005	590404..590577	-	174	WP_012054673.1	DUF5363 family protein	-
DV384_RS02915	590574..592418	-	1845	WP_114934486.1	tRNA(Met) cytidine acetyltransferase TmcA	-
DV384_RS02920	592423..592764	-	342	WP_005652996.1	SirB2 family protein	-
DV384_RS02925	592826..594496	-	1671	WP_114934487.1	energy-dependent translational throttle protein EttA	-
DV384_RS02930	594659..594982	-	324	WP_005650217.1	HigA family addiction module antidote protein	Antitoxin
DV384_RS02935	594993..595298	-	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DV384_RS02940	595623..596243	+	621	WP_005650213.1	zinc transporter binding subunit ZevA	-
DV384_RS02945	596246..597214	+	969	WP_005650211.1	zinc transporter permease subunit ZevB	-
DV384_RS02955	597829..599868	+	2040	WP_114934488.1	excinuclease ABC subunit B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T108694 WP_005650215.1 NZ_CP031241:c595298-594993 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T108694 NZ_CP031241:c595298-594993 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCCATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT108694 WP_005650217.1 NZ_CP031241:c594982-594659 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT108694 NZ_CP031241:c594982-594659 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACACTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAACCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70