Detailed information of TA system TA108678

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 734524..735163
• Replicon: chromosome
• Accession: NZ_CP031237
• Organism: Haemophilus influenzae strain M12125

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: DV365_RS03575
• Protein ID: WP_005650215.1
• Coordinates: 734524..734829 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DV365_RS03580
• Protein ID: WP_005650217.1
• Coordinates: 734840..735163 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DV365_RS03555	729954..731993	-	2040	WP_071163269.1	excinuclease ABC subunit B	-
DV365_RS03565	732608..733576	-	969	WP_114890278.1	zinc transporter permease subunit ZevB	-
DV365_RS03570	733579..734199	-	621	WP_005650213.1	zinc transporter binding subunit ZevA	-
DV365_RS03575	734524..734829	+	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DV365_RS03580	734840..735163	+	324	WP_005650217.1	HigA family addiction module antidote protein	Antitoxin
DV365_RS03585	735326..736996	+	1671	WP_114890279.1	energy-dependent translational throttle protein EttA	-
DV365_RS03590	737058..737399	+	342	WP_005666735.1	SirB2 family protein	-
DV365_RS03595	737404..739248	+	1845	WP_114890280.1	tRNA(Met) cytidine acetyltransferase TmcA	-
DV365_RS08925	739245..739418	+	174	WP_005657572.1	DUF5363 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T108678 WP_005650215.1 NZ_CP031237:734524-734829 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T108678 NZ_CP031237:734524-734829 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCCATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT108678 WP_005650217.1 NZ_CP031237:734840-735163 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT108678 NZ_CP031237:734840-735163 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70