Detailed information of TA system TA108675

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA-HipA
• Location: 1850633..1851981
• Replicon: chromosome
• Accession: NZ_CP031236
• Organism: Haemophilus influenzae strain M25267

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: A0A8G1T5W4
• Locus tag: DV400_RS09175
• Protein ID: WP_061671425.1
• Coordinates: 1850950..1851981 (+)
• Length: 344 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: Q4QMQ1
• Locus tag: DV400_RS09170
• Protein ID: WP_011272158.1
• Coordinates: 1850633..1850953 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DV400_RS09125	1845877..1846155	+	279	WP_050948950.1	cell division protein FtsB	-
DV400_RS09130	1846155..1846832	+	678	WP_050948951.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
DV400_RS09135	1846829..1847305	+	477	WP_005658971.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
DV400_RS09615	1847349..1847516	-	168	WP_171970165.1	hypothetical protein	-
DV400_RS09145	1847782..1848216	-	435	WP_005658975.1	D-tyrosyl-tRNA(Tyr) deacylase	-
DV400_RS09150	1848213..1848653	-	441	WP_050948953.1	FMN-binding protein MioC	-
DV400_RS09155	1848751..1848969	-	219	WP_005650820.1	cell division protein ZapB	-
DV400_RS09160	1849131..1850132	+	1002	WP_050948954.1	class II fructose-bisphosphatase	-
DV400_RS09165	1850337..1850645	+	309	WP_050948955.1	helix-turn-helix domain-containing protein	-
DV400_RS09170	1850633..1850953	+	321	WP_011272158.1	type II toxin-antitoxin system HipA family toxin	Antitoxin
DV400_RS09175	1850950..1851981	+	1032	WP_061671425.1	type II toxin-antitoxin system HipA family toxin	Toxin
DV400_RS09180	1852064..1853722	-	1659	WP_050948956.1	thiol reductant ABC exporter subunit CydC	-
DV400_RS09185	1853715..1855460	-	1746	WP_044345224.1	ABC transporter ATP-binding protein/permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (56-290)

Antitoxin

• HipA, N-terminal subdomain 1 (9-101)

Sequences

Toxin

Protein

• Length: 344 a.a.
• Molecular weight: 39891.44 Da
• Isoelectric Point: 6.5050

>T108675 WP_061671425.1 NZ_CP031236:1850950-1851981 [Haemophilus influenzae]
MNFCRILLKPLKKNEVHSGYSTKGLHYLTGNKHFNPELPFSRQEFITVKPQKQQGMSISGFQPKLQLIIKDDHFDSVDQQ
GHYILKPSPEEYPFLAENEHATMRIMKELGFDVPENGLVSFAGEQNQKEFAFVIKRFDRDEQQKPMHQEQLDGAMNIRDK
YGKIGTDNEQYVSYEQIAKFILQHTENHLAQQQEIFRRIIYAYLLGNNDLHLRNFSFIYPKNSHPKLAPIYDFVSVSPYP
EIFNSTLLALQLLAREEGNATLAKGFNTQYGEYIGDDFVEFGENIGLNKNVIIQKLIPEITQEKEKVEQIYSQSFMPQPH
IDCVLKTYRKRLALLNVLNEPEL

Nucleotide

• Length: 1032 bp

>T108675 NZ_CP031236:1850950-1851981 [Haemophilus influenzae]
ATGAATTTTTGTCGTATTTTATTAAAGCCATTGAAGAAAAATGAAGTTCATTCTGGTTACAGCACAAAAGGTTTACACTA
TCTTACAGGCAATAAACATTTTAATCCTGAATTGCCCTTTTCTCGCCAAGAATTTATTACAGTGAAACCCCAAAAACAGC
AAGGGATGAGCATTTCAGGTTTTCAACCAAAATTACAGCTTATTATCAAAGATGATCACTTTGACAGCGTAGATCAACAG
GGACATTACATTTTAAAGCCTTCGCCCGAAGAGTATCCTTTTTTAGCTGAAAATGAACACGCCACCATGCGCATTATGAA
AGAATTAGGGTTTGATGTGCCAGAAAATGGTTTAGTTTCTTTTGCTGGCGAACAAAATCAGAAGGAATTTGCTTTTGTTA
TAAAGCGTTTTGATCGTGATGAACAGCAAAAACCAATGCACCAAGAACAACTTGATGGTGCAATGAATATTCGAGACAAG
TACGGAAAAATAGGTACTGATAATGAGCAATATGTGAGTTATGAACAAATTGCTAAGTTTATTTTACAACACACTGAAAA
TCATTTAGCTCAGCAACAAGAGATTTTCCGTCGAATTATTTACGCTTATTTATTAGGAAATAATGATCTTCACTTACGCA
ATTTTTCATTTATTTATCCCAAAAATAGTCATCCAAAACTTGCTCCAATTTATGACTTTGTATCGGTGTCGCCCTATCCT
GAAATATTTAATTCAACACTACTTGCTTTGCAATTGTTAGCACGAGAAGAGGGTAATGCCACACTCGCCAAAGGATTTAA
TACTCAATACGGTGAATATATCGGCGATGATTTTGTCGAGTTTGGGGAAAATATTGGATTAAATAAAAATGTGATTATTC
AAAAATTAATACCTGAAATTACACAAGAAAAGGAAAAGGTGGAGCAGATTTATTCACAATCCTTTATGCCGCAACCACAT
ATTGATTGCGTACTGAAAACTTATCGTAAGCGTTTAGCTTTGCTTAACGTATTAAATGAACCTGAGCTATAA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12437.12 Da
• Isoelectric Point: 5.9670

>AT108675 WP_011272158.1 NZ_CP031236:1850633-1850953 [Haemophilus influenzae]
MRDLVRSGKVFLYGEFIGLLREDHRGFHFAYNPDYQGIPLSLSFPIEQSPFHSDTLFPYFASLVPEGWLKHKYALHQRID
ESDMFRFLLNNGENMLGAVQIQEEKQ

Nucleotide

• Length: 321 bp

>AT108675 NZ_CP031236:1850633-1850953 [Haemophilus influenzae]
ATGCGCGATTTAGTCCGCTCGGGAAAAGTATTTCTCTACGGCGAATTTATTGGCTTACTTAGAGAAGATCATCGTGGTTT
TCATTTTGCTTACAATCCCGATTATCAAGGAATACCGCTCTCTCTCAGCTTTCCTATTGAGCAAAGTCCTTTTCATTCTG
ACACCTTATTCCCTTATTTTGCATCCCTCGTCCCCGAAGGCTGGCTAAAACACAAATATGCACTTCATCAACGAATTGAT
GAAAGCGATATGTTTCGCTTTTTACTTAATAACGGGGAAAATATGTTAGGTGCAGTGCAAATTCAGGAGGAAAAACAATG
A

Structures

Antitoxin

• AlphaFold DB: Q4QMQ1