Detailed information of TA system TA108663

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2287..2541
• Replicon: plasmid pEsST410_NW_3
• Accession: NZ_CP031233
• Organism: Escherichia coli strain Es_ST410_NW1_NDM_09_2017

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: DVH14_RS23975
• Protein ID: WP_015387399.1
• Coordinates: 2287..2493 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	2480..2541 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DVH14_RS23950	569..1051	+	483	Protein_0	VENN motif pre-toxin domain-containing protein	-
DVH14_RS23955	997..1443	-	447	Protein_1	incFII family plasmid replication initiator RepA	-
DVH14_RS23960	1436..1510	-	75	WP_032336874.1	RepA leader peptide Tap	-
DVH14_RS23970	1746..2003	-	258	WP_000083833.1	replication regulatory protein RepA	-
DVH14_RS23975	2287..2493	-	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2480..2541	+	62	NuclAT_0	-	Antitoxin
-	2480..2541	+	62	NuclAT_0	-	Antitoxin
-	2480..2541	+	62	NuclAT_0	-	Antitoxin
-	2480..2541	+	62	NuclAT_0	-	Antitoxin
DVH14_RS23985	2680..2862	-	183	WP_000968309.1	hypothetical protein	-
DVH14_RS23990	2963..3579	+	617	Protein_6	IS1 family transposase	-
DVH14_RS23995	3617..5188	-	1572	WP_023149734.1	IS66 family transposase	-
DVH14_RS25755	5208..5324	-	117	Protein_8	IS66 family insertion sequence element accessory protein TnpB	-
DVH14_RS24005	5375..7420	-	2046	Protein_9	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sitABCD / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / qepA1	iutA / iucD / iucC / iucB / iucA	1..98308	98308

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T108663 WP_015387399.1 NZ_CP031233:c2493-2287 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T108663 NZ_CP031233:c2493-2287 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT108663 NZ_CP031233:2480-2541 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin