Detailed information of TA system TA108656

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3832485..3833212
• Replicon: chromosome
• Accession: NZ_CP031231
• Organism: Escherichia coli strain Es_ST410_NW1_NDM_09_2017

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: DVH14_RS18765
• Protein ID: WP_000547564.1
• Coordinates: 3832901..3833212 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DVH14_RS18760
• Protein ID: WP_000126294.1
• Coordinates: 3832485..3832904 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DVH14_RS18740	3827535..3828062	-	528	WP_001078777.1	electron transport protein HydN	-
DVH14_RS18745	3828211..3829221	-	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
DVH14_RS18750	3829481..3830938	+	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
DVH14_RS18755	3830947..3832371	+	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
DVH14_RS18760	3832485..3832904	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
DVH14_RS18765	3832901..3833212	-	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
DVH14_RS18770	3833386..3834147	-	762	WP_001026446.1	hypothetical protein	-
DVH14_RS18775	3834172..3834642	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
DVH14_RS18780	3834635..3835045	-	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
DVH14_RS18785	3835042..3835809	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
DVH14_RS18790	3835809..3836351	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
DVH14_RS18795	3836361..3838070	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T108656 WP_000547564.1 NZ_CP031231:c3833212-3832901 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T108656 NZ_CP031231:c3833212-3832901 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT108656 WP_000126294.1 NZ_CP031231:c3832904-3832485 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT108656 NZ_CP031231:c3832904-3832485 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5