Detailed information of TA system TA108619

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3382066..3382668
• Replicon: chromosome
• Accession: NZ_CP031215
• Organism: Escherichia coli strain Es_ST80_L1_NDM_10_2017

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: DV870_RS17475
• Protein ID: WP_000897302.1
• Coordinates: 3382357..3382668 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DV870_RS17470
• Protein ID: WP_000356397.1
• Coordinates: 3382066..3382356 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DV870_RS17435	3378139..3379041	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
DV870_RS17440	3379038..3379673	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
DV870_RS17445	3379670..3380599	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
DV870_RS17450	3380815..3381033	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
DV870_RS17460	3381429..3381707	-	279	WP_001296612.1	hypothetical protein	-
DV870_RS17470	3382066..3382356	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
DV870_RS17475	3382357..3382668	-	312	WP_000897302.1	hypothetical protein	Toxin
DV870_RS17480	3382897..3383805	+	909	WP_001331553.1	alpha/beta hydrolase	-
DV870_RS17485	3383869..3384810	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
DV870_RS17490	3384855..3385292	-	438	WP_021531734.1	D-tyrosyl-tRNA(Tyr) deacylase	-
DV870_RS17495	3385289..3386161	-	873	WP_000920762.1	virulence factor BrkB family protein	-
DV870_RS17500	3386155..3386754	-	600	WP_001314324.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T108619 WP_000897302.1 NZ_CP031215:c3382668-3382357 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T108619 NZ_CP031215:c3382668-3382357 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT108619 WP_000356397.1 NZ_CP031215:c3382356-3382066 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT108619 NZ_CP031215:c3382356-3382066 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416