Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3630813..3631507 Replicon chromosome
Accession NZ_CP031214
Organism Escherichia coli strain C600

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag DK885_RS18960 Protein ID WP_001263489.1
Coordinates 3630813..3631211 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag DK885_RS18965 Protein ID WP_000554758.1
Coordinates 3631214..3631507 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3626401..3626481 - 81 NuclAT_12 - -
- 3626401..3626481 - 81 NuclAT_12 - -
- 3626401..3626481 - 81 NuclAT_12 - -
- 3626401..3626481 - 81 NuclAT_12 - -
DK885_RS18930 3627077..3627535 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
DK885_RS18935 3627796..3629253 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
DK885_RS18940 3629310..3629831 - 522 Protein_3460 peptide chain release factor H -
DK885_RS18945 3629827..3630033 - 207 Protein_3461 RtcB family protein -
DK885_RS18955 3630351..3630803 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
DK885_RS18960 3630813..3631211 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
DK885_RS18965 3631214..3631507 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
DK885_RS18970 3631559..3632614 - 1056 WP_001226164.1 DNA polymerase IV -
DK885_RS18975 3632685..3633470 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
DK885_RS18980 3633442..3635154 + 1713 Protein_3467 flagellar biosynthesis protein FlhA -
DK885_RS18985 3635378..3635875 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T108588 WP_001263489.1 NZ_CP031214:c3631211-3630813 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T108588 NZ_CP031214:c3631211-3630813 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT108588 WP_000554758.1 NZ_CP031214:c3631507-3631214 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT108588 NZ_CP031214:c3631507-3631214 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References