Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1833580..1834082 Replicon chromosome
Accession NZ_CP031214
Organism Escherichia coli strain C600

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag DK885_RS09265 Protein ID WP_000767829.1
Coordinates 1833828..1834082 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DK885_RS09260 Protein ID WP_001259255.1
Coordinates 1833580..1833831 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DK885_RS09230 1828758..1829825 - 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
DK885_RS09235 1829825..1830895 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
DK885_RS09240 1830892..1832196 - 1305 WP_000009594.1 histidinol dehydrogenase -
DK885_RS09245 1832202..1833101 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
DK885_RS09255 1833247..1833297 - 51 WP_001364200.1 his operon leader peptide -
DK885_RS09260 1833580..1833831 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DK885_RS09265 1833828..1834082 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DK885_RS09270 1834165..1834989 + 825 WP_000754737.1 SDR family oxidoreductase -
DK885_RS09275 1835035..1835964 + 930 WP_000803351.1 LysR family transcriptional regulator -
DK885_RS09280 1836179..1836241 + 63 WP_010723108.1 membrane protein YoeI -
DK885_RS09285 1836231..1837589 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
DK885_RS09290 1837768..1838826 + 1059 WP_000492339.1 transport protein YeeE -
DK885_RS09295 1838840..1839067 + 228 WP_000234896.1 sulfurtransferase TusA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T108573 WP_000767829.1 NZ_CP031214:1833828-1834082 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T108573 NZ_CP031214:1833828-1834082 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT108573 WP_001259255.1 NZ_CP031214:1833580-1833831 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT108573 NZ_CP031214:1833580-1833831 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References