Detailed information of TA system TA108543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-YefM
• Location: 3069910..3070415
• Replicon: chromosome
• Accession: NZ_CP031145
• Organism: Intrasporangium calvum strain C5

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E6SBE3
• Locus tag: DN585_RS14170
• Protein ID: WP_013493783.1
• Coordinates: 3069910..3070176 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: DN585_RS14175
• Protein ID: WP_041308866.1
• Coordinates: 3070173..3070415 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DN585_RS14155	3066916..3067374	-	459	WP_148236625.1	hypothetical protein	-
DN585_RS14160	3068695..3069132	+	438	WP_199921537.1	SigE family RNA polymerase sigma factor	-
DN585_RS18960	3069136..3069309	+	174	WP_013493781.1	hypothetical protein	-
DN585_RS14165	3069470..3069805	+	336	WP_013493782.1	hypothetical protein	-
DN585_RS14170	3069910..3070176	-	267	WP_013493783.1	Txe/YoeB family addiction module toxin	Toxin
DN585_RS14175	3070173..3070415	-	243	WP_041308866.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
DN585_RS14180	3071088..3071369	+	282	WP_013493785.1	hypothetical protein	-
DN585_RS14185	3071606..3072529	-	924	WP_150132621.1	hypothetical protein	-
DN585_RS14190	3072721..3073281	-	561	WP_052338031.1	IS110 family transposase	-
DN585_RS18965	3073358..3073702	-	345	WP_199921538.1	transposase	-
DN585_RS14200	3073842..3074231	-	390	WP_199921539.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3060116..3072511	12395

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (7-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-68)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10250.82 Da
• Isoelectric Point: 9.7537

>T108543 WP_013493783.1 NZ_CP031145:c3070176-3069910 [Intrasporangium calvum]
VSAWTLIYSRQAQKDAKKLASSGLKTKAQRLLDVISEDPFTTPPRYEKLVGDLAGCYSRRINIQHRLVYEVLPDQHVVHI
LRMWTHYE

Nucleotide

• Length: 267 bp

>T108543 NZ_CP031145:c3070176-3069910 [Intrasporangium calvum]
GTGAGCGCCTGGACGCTGATCTATTCGCGCCAAGCTCAGAAGGACGCCAAGAAGCTGGCATCCTCCGGGTTGAAGACCAA
GGCCCAGCGTCTGCTCGACGTCATCAGCGAGGATCCATTCACCACGCCGCCGCGGTACGAGAAACTGGTCGGCGACCTGG
CGGGCTGCTACTCCCGGCGCATCAACATCCAACACCGCCTCGTCTACGAGGTCCTTCCCGACCAGCACGTCGTCCACATC
CTGCGCATGTGGACGCACTACGAATAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8614.50 Da
• Isoelectric Point: 3.8807

>AT108543 WP_041308866.1 NZ_CP031145:c3070415-3070173 [Intrasporangium calvum]
VTAVSATTARANLYRLIDQVNDESEPLTITGQRGNAVLVGEEDWQAIQETLFLASVPGLAQSIREARAEGIEAGSTELDW

Nucleotide

• Length: 243 bp

>AT108543 NZ_CP031145:c3070415-3070173 [Intrasporangium calvum]
GTGACTGCAGTGAGCGCGACGACGGCACGGGCCAACCTGTACCGCCTGATCGACCAGGTCAACGACGAGTCTGAGCCGCT
GACCATTACCGGGCAGCGGGGCAACGCGGTGCTGGTCGGGGAGGAGGACTGGCAGGCGATCCAGGAGACGCTGTTCTTGG
CCTCCGTCCCGGGTCTGGCCCAGTCCATCCGCGAGGCCCGCGCCGAGGGTATCGAGGCCGGGTCCACCGAGCTCGACTGG
TGA

Structures

Toxin

• AlphaFold DB: E6SBE3