Detailed information of TA system TA108477

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 82195..82459
• Replicon: plasmid pAMSC6
• Accession: NZ_CP031111
• Organism: Escherichia coli strain AMSCJX02

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: DUT84_RS28225
• Protein ID: WP_001331364.1
• Coordinates: 82307..82459 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	82195..82252 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DUT84_RS28210	78533..79603	-	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
DUT84_RS28215	79622..80830	-	1209	WP_001383960.1	IncI1-type conjugal transfer protein TrbA	-
DUT84_RS28220	81048..82034	-	987	WP_001257838.1	hypothetical protein	-
-	82195..82252	-	58	NuclAT_0	-	Antitoxin
-	82195..82252	-	58	NuclAT_0	-	Antitoxin
-	82195..82252	-	58	NuclAT_0	-	Antitoxin
-	82195..82252	-	58	NuclAT_0	-	Antitoxin
DUT84_RS28225	82307..82459	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
DUT84_RS28230	82531..82782	-	252	WP_001291964.1	hypothetical protein	-
DUT84_RS28900	83441..83617	-	177	WP_001054900.1	hypothetical protein	-
DUT84_RS28235	83949..84158	+	210	WP_000062602.1	HEAT repeat domain-containing protein	-
DUT84_RS28240	84230..84892	-	663	WP_000644792.1	plasmid IncI1-type surface exclusion protein ExcA	-
DUT84_RS28245	84957..87125	-	2169	WP_077250315.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..95328	95328

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T108477 WP_001331364.1 NZ_CP031111:82307-82459 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T108477 NZ_CP031111:82307-82459 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 58 bp

>AT108477 NZ_CP031111:c82252-82195 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin