Detailed information of TA system TA108467

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 55996..56422
• Replicon: plasmid pAMSC2
• Accession: NZ_CP031107
• Organism: Escherichia coli strain AMSCJX02

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: DUT84_RS25905
• Protein ID: WP_001302184.1
• Coordinates: 55996..56154 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	56198..56422 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DUT84_RS25870	51169..51564	-	396	WP_001309237.1	conjugal transfer relaxosome protein TraY	-
DUT84_RS25875	51663..52352	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
DUT84_RS25880	52539..52922	-	384	WP_001151524.1	relaxosome protein TraM	-
DUT84_RS25885	53243..53845	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
DUT84_RS25890	54142..54963	-	822	WP_001234487.1	DUF945 domain-containing protein	-
DUT84_RS25895	55074..55370	-	297	WP_001272245.1	hypothetical protein	-
DUT84_RS25905	55996..56154	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	56198..56422	-	225	NuclAT_0	-	Antitoxin
-	56198..56422	-	225	NuclAT_0	-	Antitoxin
-	56198..56422	-	225	NuclAT_0	-	Antitoxin
-	56198..56422	-	225	NuclAT_0	-	Antitoxin
DUT84_RS25910	56434..57153	-	720	WP_001276236.1	plasmid SOS inhibition protein A	-
DUT84_RS25915	57150..57584	-	435	WP_000845962.1	conjugation system SOS inhibitor PsiB	-
DUT84_RS25920	57653..59617	-	1965	WP_068862930.1	ParB/RepB/Spo0J family partition protein	-
DUT84_RS25925	59681..59914	-	234	WP_000005988.1	DUF905 family protein	-
DUT84_RS25930	59877..60509	-	633	WP_039023645.1	single-stranded DNA-binding protein	-
DUT84_RS25940	60810..61235	+	426	WP_015387398.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat	1..203856	203856

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T108467 WP_001302184.1 NZ_CP031107:c56154-55996 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T108467 NZ_CP031107:c56154-55996 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT108467 NZ_CP031107:c56422-56198 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGATTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin