Detailed information of TA system TA108462

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 17897..18151
• Replicon: plasmid pAMSC2
• Accession: NZ_CP031107
• Organism: Escherichia coli strain AMSCJX02

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: DUT84_RS25680
• Protein ID: WP_001351576.1
• Coordinates: 17897..18103 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	18090..18151 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DUT84_RS25625	13436..13783	+	348	Protein_11	IS1-like element IS1A family transposase	-
DUT84_RS25635	14223..14510	-	288	WP_000222760.1	type II toxin-antitoxin system RelE/ParE family toxin	-
DUT84_RS25640	14507..14758	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
DUT84_RS25655	15725..16582	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
DUT84_RS25660	16575..17057	-	483	WP_001273588.1	hypothetical protein	-
DUT84_RS25665	17050..17124	-	75	WP_001442103.1	RepA leader peptide Tap	-
DUT84_RS25675	17356..17613	-	258	WP_000083845.1	replication regulatory protein RepA	-
DUT84_RS25680	17897..18103	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	18090..18151	+	62	NuclAT_1	-	Antitoxin
-	18090..18151	+	62	NuclAT_1	-	Antitoxin
-	18090..18151	+	62	NuclAT_1	-	Antitoxin
-	18090..18151	+	62	NuclAT_1	-	Antitoxin
DUT84_RS28800	18407..18481	-	75	Protein_19	endonuclease	-
DUT84_RS25690	18727..18939	-	213	WP_001299730.1	ANR family transcriptional regulator	-
DUT84_RS25695	19075..19635	-	561	WP_032072881.1	fertility inhibition protein FinO	-
DUT84_RS25700	19738..20598	-	861	WP_000704513.1	alpha/beta hydrolase	-
DUT84_RS25705	20657..21403	-	747	WP_114455236.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat	1..203856	203856

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T108462 WP_001351576.1 NZ_CP031107:c18103-17897 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T108462 NZ_CP031107:c18103-17897 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT108462 NZ_CP031107:18090-18151 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin