Detailed information of TA system TA108460

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 94339..94996
• Replicon: plasmid pAMSC1
• Accession: NZ_CP031106
• Organism: Escherichia coli strain AMSCJX02

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: DUT84_RS25300
• Protein ID: WP_000270043.1
• Coordinates: 94339..94689 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DUT84_RS25305
• Protein ID: WP_000124640.1
• Coordinates: 94694..94996 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DUT84_RS25250	89650..89829	+	180	Protein_102	winged helix-turn-helix domain-containing protein	-
DUT84_RS25255	89894..90598	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
DUT84_RS25260	90813..91310	-	498	WP_000062185.1	hypothetical protein	-
DUT84_RS25265	91313..91801	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
DUT84_RS25270	91898..92233	+	336	WP_000683476.1	hypothetical protein	-
DUT84_RS25275	92248..92718	-	471	WP_001281821.1	hypothetical protein	-
DUT84_RS25280	92711..93082	-	372	WP_000516916.1	hypothetical protein	-
DUT84_RS25285	93093..93287	-	195	WP_000343597.1	hypothetical protein	-
DUT84_RS25295	93628..94176	-	549	WP_001061574.1	transcriptional regulator	-
DUT84_RS25300	94339..94689	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DUT84_RS25305	94694..94996	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
DUT84_RS25310	95023..95316	-	294	WP_001239997.1	chromosome segregation protein ParM	-
DUT84_RS25315	95404..95676	-	273	WP_001043047.1	HU family DNA-binding protein	-
DUT84_RS25320	95734..96261	-	528	WP_001236377.1	thermonuclease family protein	-
DUT84_RS25325	96278..96466	-	189	WP_001270411.1	hypothetical protein	-
DUT84_RS25330	96492..97349	-	858	WP_001167032.1	hypothetical protein	-
DUT84_RS25335	97336..97566	-	231	WP_000972663.1	hypothetical protein	-
DUT84_RS25340	97566..98084	-	519	WP_000210756.1	nitrite reductase	-
DUT84_RS25345	98081..98527	-	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
DUT84_RS25350	98527..98886	-	360	WP_000422768.1	hypothetical protein	-
DUT84_RS25355	98943..99371	-	429	WP_000591074.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR	-	1..131411	131411

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T108460 WP_000270043.1 NZ_CP031106:94339-94689 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T108460 NZ_CP031106:94339-94689 [Escherichia coli]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT108460 WP_000124640.1 NZ_CP031106:94694-94996 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT108460 NZ_CP031106:94694-94996 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9