Detailed information of TA system TA108454

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 4490119..4490490
• Replicon: chromosome
• Accession: NZ_CP031105
• Organism: Escherichia coli strain AMSCJX02

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: DUT84_RS23155
• Protein ID: WP_001317028.1
• Coordinates: 4490296..4490490 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	4490119..4490297 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DUT84_RS23130	4486074..4487447	+	1374	WP_000123719.1	ATP-dependent RNA helicase DbpA	-
DUT84_RS23135	4487576..4488511	-	936	WP_001157408.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
DUT84_RS23140	4488563..4489798	-	1236	WP_000040858.1	site-specific integrase	-
DUT84_RS23145	4489800..4490015	-	216	WP_000079604.1	excisionase XisR	-
DUT84_RS23150	4490094..4490303	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	4490119..4490297	+	179	NuclAT_0	-	Antitoxin
-	4490119..4490297	+	179	NuclAT_0	-	Antitoxin
-	4490119..4490297	+	179	NuclAT_0	-	Antitoxin
-	4490119..4490297	+	179	NuclAT_0	-	Antitoxin
DUT84_RS23155	4490296..4490490	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
DUT84_RS23160	4490547..4491356	-	810	WP_000166319.1	recombination protein RecT	-
DUT84_RS23165	4491349..4493949	-	2601	WP_000105139.1	exodeoxyribonuclease VIII	-
DUT84_RS23170	4494051..4494326	-	276	WP_000632297.1	protein RacC	-
DUT84_RS23175	4494401..4494571	-	171	WP_001352098.1	conserved protein, Rac prophage	-
DUT84_RS23180	4494571..4494792	-	222	WP_112021577.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4488563..4517261	28698

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T108454 WP_001317028.1 NZ_CP031105:c4490490-4490296 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T108454 NZ_CP031105:c4490490-4490296 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT108454 NZ_CP031105:4490119-4490297 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin