Detailed information of TA system TA108414

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 29520..30172
• Replicon: plasmid pW17-2
• Accession: NZ_CP031103
• Organism: Leclercia sp. W17

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: DVA44_RS25130
• Protein ID: WP_114388501.1
• Coordinates: 29933..30172 (-)
• Length: 80 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DVA44_RS25125
• Protein ID: WP_114388500.1
• Coordinates: 29520..29936 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DVA44_RS25085	24890..25180	-	291	WP_114388493.1	hypothetical protein	-
DVA44_RS25090	25607..25927	-	321	WP_114388494.1	helix-turn-helix domain-containing protein	-
DVA44_RS25095	25920..26198	-	279	WP_139157131.1	hypothetical protein	-
DVA44_RS25100	26575..27165	-	591	WP_139157132.1	hypothetical protein	-
DVA44_RS25105	27162..27815	-	654	WP_114388497.1	VRR-NUC domain-containing protein	-
DVA44_RS25110	28696..28875	+	180	WP_114388498.1	MbcA/ParS/Xre antitoxin family protein	-
DVA44_RS25120	29192..29431	-	240	WP_114388499.1	hypothetical protein	-
DVA44_RS25125	29520..29936	-	417	WP_114388500.1	transcriptional regulator	Antitoxin
DVA44_RS25130	29933..30172	-	240	WP_114388501.1	type II toxin-antitoxin system HigB family toxin	Toxin
DVA44_RS25135	30256..30953	-	698	Protein_35	IS1 family transposase	-
DVA44_RS25140	30982..31329	-	348	WP_114388502.1	hypothetical protein	-
DVA44_RS25145	31341..31915	-	575	Protein_37	transposase	-
DVA44_RS25150	32038..32358	+	321	WP_114388503.1	hypothetical protein	-
DVA44_RS25155	32397..32702	-	306	WP_114388504.1	Lar family restriction alleviation protein	-
DVA44_RS25160	33050..33667	+	618	WP_114388505.1	ParA family protein	-
DVA44_RS25165	33720..33959	+	240	WP_114388506.1	DNA partition complex ParG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..92616	92616

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (3-67)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-136)

Sequences

Toxin

Protein

• Length: 80 a.a.
• Molecular weight: 9320.82 Da
• Isoelectric Point: 10.4324

>T108414 WP_114388501.1 NZ_CP031103:c30172-29933 [Leclercia sp. W17]
MAKTIEKGSFATPEDLRKFFRSLDNHKDLNRHYIIDIGHNEWRAVLKIDFVNQIVFVKGIFTHKEYDVFNAKAKKGKIK

Nucleotide

• Length: 240 bp

>T108414 NZ_CP031103:c30172-29933 [Leclercia sp. W17]
ATGGCTAAGACGATAGAAAAGGGCTCTTTCGCAACGCCGGAAGACCTAAGAAAATTTTTCCGCTCGTTGGACAACCATAA
GGATCTGAATAGACACTACATTATCGATATCGGTCATAACGAATGGCGAGCTGTTTTGAAAATCGACTTTGTTAATCAAA
TCGTTTTCGTAAAAGGTATTTTTACTCATAAAGAGTATGACGTATTCAACGCCAAAGCGAAGAAAGGAAAGATAAAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 14954.15 Da
• Isoelectric Point: 4.7590

>AT108414 WP_114388500.1 NZ_CP031103:c29936-29520 [Leclercia sp. W17]
MNMPEITKAGAALFAVAPFIAGIHTEEQHAAALEFVENLFDANDTDNPLFEIVAAKIKEYESTAPEYAEFNARLAAIPTG
VAVLRTLISQHDLTFSDLPEIGGKSMVSRVLKGERKLSLDHIKALAARFGISPALFID

Nucleotide

• Length: 417 bp

>AT108414 NZ_CP031103:c29936-29520 [Leclercia sp. W17]
ATGAACATGCCAGAAATCACTAAAGCAGGCGCGGCGCTTTTTGCCGTTGCGCCTTTCATCGCTGGGATTCATACCGAAGA
ACAGCACGCCGCCGCGCTGGAATTTGTAGAAAATCTTTTCGACGCGAACGATACAGACAACCCACTCTTTGAGATCGTCG
CTGCGAAAATTAAAGAATACGAAAGTACCGCGCCGGAATATGCAGAATTTAACGCGCGATTAGCCGCGATCCCTACAGGC
GTTGCGGTATTACGCACTCTTATAAGCCAACATGATTTAACATTCTCCGACTTACCGGAGATTGGCGGAAAATCGATGGT
ATCTCGCGTGCTTAAAGGCGAGCGTAAATTATCATTAGACCATATCAAAGCGCTGGCAGCGCGCTTTGGAATTTCACCTG
CGCTTTTCATCGATTAA