Detailed information of TA system TA108397

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3422066..3422637
• Replicon: chromosome
• Accession: NZ_CP031093
• Organism: Hydrocarboniclastica marina strain soil36-7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: soil367_RS15075
• Protein ID: WP_136549874.1
• Coordinates: 3422066..3422344 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: soil367_RS15080
• Protein ID: WP_136549875.1
• Coordinates: 3422344..3422637 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
soil367_RS15055	3417454..3418422	+	969	WP_136549870.1	ATP-binding protein	-
soil367_RS15060	3418458..3420941	+	2484	WP_136549871.1	S8 family peptidase	-
soil367_RS15065	3421212..3421700	+	489	WP_136549872.1	hypothetical protein	-
soil367_RS15070	3421714..3422004	+	291	WP_136549873.1	hypothetical protein	-
soil367_RS15075	3422066..3422344	+	279	WP_136549874.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
soil367_RS15080	3422344..3422637	+	294	WP_136549875.1	HigA family addiction module antidote protein	Antitoxin
soil367_RS15085	3422951..3423187	+	237	WP_136549876.1	hypothetical protein	-
soil367_RS15090	3423536..3423814	+	279	WP_136549877.1	type II toxin-antitoxin system RelE/ParE family toxin	-
soil367_RS15095	3423827..3424126	+	300	WP_172962364.1	HigA family addiction module antidote protein	-
soil367_RS15100	3424256..3424414	-	159	Protein_2967	type II toxin-antitoxin system YoeB family toxin	-
soil367_RS15105	3424413..3424649	-	237	WP_136549878.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
soil367_RS15110	3424889..3427018	+	2130	WP_136549879.1	TonB-dependent receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3401192..3671756	270564

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-83)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10596.00 Da
• Isoelectric Point: 9.4399

>T108397 WP_136549874.1 NZ_CP031093:3422066-3422344 [Hydrocarboniclastica marina]
MIKSFRHKGLKRFYATGNTAGIQSDHAKKLRLQLAALDTAASVTDMDIPGFRLHPMKGSDKGRWSIWVNGNWRMTFEFED
GNAYVLDYEDYH

Nucleotide

• Length: 279 bp

>T108397 NZ_CP031093:3422066-3422344 [Hydrocarboniclastica marina]
ATGATTAAATCATTCCGTCATAAAGGGCTAAAGCGCTTTTACGCGACTGGCAATACCGCTGGCATACAATCAGATCACGC
CAAGAAACTGCGCTTGCAGCTTGCGGCCCTCGATACAGCCGCTTCAGTGACGGATATGGACATCCCCGGTTTCCGGCTTC
ATCCGATGAAGGGGAGCGACAAAGGACGATGGTCAATCTGGGTTAACGGAAACTGGCGCATGACGTTCGAGTTTGAGGAC
GGCAACGCTTACGTTCTTGATTATGAGGATTACCACTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10842.42 Da
• Isoelectric Point: 5.6643

>AT108397 WP_136549875.1 NZ_CP031093:3422344-3422637 [Hydrocarboniclastica marina]
MTLHNPPHPGEFIREVYLEPFGISSRQLASSLGVSPSTLSRLLKGDSGISPEMSLRLSKVLGRTPESWLAMQDMYDLWLA
RRTVDVDSLRPLDFEAA

Nucleotide

• Length: 294 bp

>AT108397 NZ_CP031093:3422344-3422637 [Hydrocarboniclastica marina]
ATGACCTTGCATAATCCGCCGCATCCCGGTGAATTCATTCGGGAGGTGTACCTGGAACCTTTCGGCATCAGTTCCCGCCA
GCTTGCTTCCAGCCTTGGCGTCTCGCCCTCCACATTGTCACGACTACTCAAAGGAGACAGTGGCATCAGCCCGGAGATGT
CCCTGCGGCTGTCCAAGGTATTGGGTCGTACTCCGGAAAGTTGGCTGGCAATGCAGGATATGTATGATCTCTGGTTGGCG
CGAAGAACGGTCGATGTGGACAGTCTCCGGCCTCTTGATTTCGAAGCAGCCTAG