Detailed information of TA system TA108344

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): fst-RNAII/Fst(toxin)
• Location: 14140..14354
• Replicon: plasmid pDEF-2
• Accession: NZ_CP031029
• Organism: Enterococcus faecalis strain TY1 isolate flatfish

Toxin (Protein)

• Gene name: fst
• Uniprot ID: -
• Locus tag: DTO64_RS16560
• Protein ID: WP_107164701.1
• Coordinates: 14140..14250 (+)
• Length: 37 a.a.

Antitoxin (RNA)

Gene name	RNAII
Locus tag	-
Coordinates	14290..14354 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DTO64_RS16540	9471..12458	+	2988	WP_000343406.1	Tn3 family transposase	-
DTO64_RS16545	12622..13542	+	921	Protein_14	excinuclease ABC subunit A	-
DTO64_RS16550	13539..13889	+	351	WP_002365943.1	hypothetical protein	-
DTO64_RS16560	14140..14250	+	111	WP_107164701.1	type I toxin-antitoxin system Fst family toxin	Toxin
-	14290..14354	-	65	-	-	Antitoxin
-	14290..14392	-	103	NuclAT_0	-	-
-	14290..14392	-	103	NuclAT_0	-	-
-	14290..14392	-	103	NuclAT_0	-	-
-	14290..14392	-	103	NuclAT_0	-	-
DTO64_RS16565	14589..15050	+	462	WP_002365946.1	hypothetical protein	-
DTO64_RS17025	15221..15511	+	291	WP_002365947.1	hypothetical protein	-
DTO64_RS16575	15615..15986	-	372	WP_000049959.1	replication-associated protein RepC	-
DTO64_RS16580	15979..16824	-	846	WP_000239313.1	AAA family ATPase	-
DTO64_RS16590	17296..18306	+	1011	WP_002365949.1	replication initiator protein A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-aph(2'')	asa1	1..58791	58791

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type I toxin-antitoxin system, toxin Ldr (1-35)

Sequences

Toxin

Protein

• Length: 37 a.a.
• Molecular weight: 4090.89 Da
• Isoelectric Point: 4.1672

>T108344 WP_107164701.1 NZ_CP031029:14140-14250 [Enterococcus faecalis]
VLFVKDLMSLVIAPIFVGLVLEMISRVLDEEDDSRK

Nucleotide

• Length: 111 bp

>T108344 NZ_CP031029:14140-14250 [Enterococcus faecalis]
GTGTTATTTGTGAAAGATTTAATGTCGTTGGTTATCGCACCAATCTTTGTAGGATTGGTTCTGGAAATGATTTCTCGTGT
GTTGGACGAGGAAGACGATAGCCGAAAGTAA

Antitoxin

Nucleotide

• Length: 65 bp

>AT108344 NZ_CP031029:c14354-14290 [Enterococcus faecalis]
AACGACATTAAATCGTACAGATAACACAAAAAGCAATCCTACGGCGAATAGGATTGCTTTTTTTT

Structures

Antitoxin