Detailed information of TA system TA108335

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 2947196..2947637
• Replicon: chromosome
• Accession: NZ_CP031027
• Organism: Enterococcus faecalis strain TY1 isolate flatfish

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: A0A1J6YG70
• Locus tag: DTO64_RS15370
• Protein ID: WP_002396786.1
• Coordinates: 2947494..2947637 (+)
• Length: 48 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	2947196..2947397 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DTO64_RS15335	2942771..2944066	-	1296	WP_002396787.1	AAA family ATPase	-
DTO64_RS15350	2944528..2946144	+	1617	WP_002358967.1	phosphatase PAP2/LCP family protein	-
DTO64_RS15355	2946499..2946621	+	123	WP_002404776.1	putative holin-like toxin	-
DTO64_RS15360	2946807..2947004	+	198	WP_002358966.1	putative holin-like toxin	-
DTO64_RS15365	2947123..2947263	+	141	WP_077143780.1	putative holin-like toxin	-
-	2947196..2947397	-	202	-	-	Antitoxin
DTO64_RS15370	2947494..2947637	+	144	WP_002396786.1	putative holin-like toxin	Toxin
DTO64_RS15375	2947832..2952523	-	4692	WP_002404775.1	WxL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (14-47)

Sequences

Toxin

Protein

• Length: 48 a.a.
• Molecular weight: 5203.19 Da
• Isoelectric Point: 8.6626

>T108335 WP_002396786.1 NZ_CP031027:2947494-2947637 [Enterococcus faecalis]
MNVSTKIYERRGLLSIAEALALMISFGSFIATLIFGILEAVKEDNKK

Nucleotide

• Length: 144 bp

>T108335 NZ_CP031027:2947494-2947637 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTTAAAGAAGACAATAAAAAATAA

Antitoxin

Nucleotide

• Length: 202 bp

>AT108335 NZ_CP031027:c2947397-2947196 [Enterococcus faecalis]
GTTTTTTTAGGTAATTGTGCTGTAATAGCAATGAAAAGAGAGATATGCGCCAACATACCTCTCTGATGTAGAGCCGTTTA
AGACGGTGACCTTTTTGATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCA
ATTTCACAATCAGCGCAATCAAAGCAATGGTAAACATACCAA

Structures

Toxin

• AlphaFold DB: A0A1J6YG70

Antitoxin