Detailed information of TA system TA108312

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 2869057..2869317
• Replicon: chromosome
• Accession: NZ_CP031027
• Organism: Enterococcus faecalis strain TY1 isolate flatfish

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: -
• Locus tag: DTO64_RS14975
• Protein ID: WP_156194032.1
• Coordinates: 2869174..2869317 (-)
• Length: 48 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	2869057..2869241 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DTO64_RS14960	2864619..2865332	-	714	WP_002354877.1	trehalose operon repressor	-
DTO64_RS14965	2865594..2868337	+	2744	Protein_2757	glycoside hydrolase family 65 protein	-
DTO64_RS14970	2868352..2869002	+	651	WP_002359015.1	beta-phosphoglucomutase	-
-	2869057..2869241	+	185	-	-	Antitoxin
DTO64_RS14975	2869174..2869317	-	144	WP_156194032.1	putative holin-like toxin	Toxin
DTO64_RS14980	2869548..2870519	-	972	WP_002381060.1	ribose-phosphate diphosphokinase	-
DTO64_RS14985	2870694..2871131	-	438	WP_002354871.1	peptide-methionine (R)-S-oxide reductase MsrB	-
DTO64_RS14990	2871264..2871818	-	555	WP_002359011.1	septum formation protein Maf	-
DTO64_RS14995	2871843..2873975	-	2133	WP_002359010.1	DNA mismatch repair endonuclease MutL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (14-47)

Sequences

Toxin

Protein

• Length: 48 a.a.
• Molecular weight: 5327.50 Da
• Isoelectric Point: 10.8331

>T108312 WP_156194032.1 NZ_CP031027:c2869317-2869174 [Enterococcus faecalis]
MSVLPKFTERRGLLSAYETIQTILGFGMFTISLIALIVKLLKNDKKK

Nucleotide

• Length: 144 bp

>T108312 NZ_CP031027:c2869317-2869174 [Enterococcus faecalis]
ATGAGCGTACTTCCAAAATTTACAGAAAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGG
TATGTTTACCATTTCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin

Nucleotide

• Length: 185 bp

>AT108312 NZ_CP031027:2869057-2869241 [Enterococcus faecalis]
TGCTAGAATGTAGATGAAAAGAGAGAGATGCGTCAACATACCTCTCTGATGTAGAGCCGTTTGAGACGGTGACCAATTGT
ATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATTTCACAATCAGCGCA
ATCAAAGAAATGGTAAACATACCAA

Structures

Antitoxin