Detailed information of TA system TA108267

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4824198..4824800
• Replicon: chromosome
• Accession: NZ_CP030939
• Organism: Escherichia coli strain AMSHJX01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: DTO54_RS25265
• Protein ID: WP_000897305.1
• Coordinates: 4824489..4824800 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DTO54_RS25260
• Protein ID: WP_000356397.1
• Coordinates: 4824198..4824488 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DTO54_RS25230	4820124..4821026	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
DTO54_RS25235	4821023..4821658	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
DTO54_RS25240	4821655..4822584	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
DTO54_RS25245	4822914..4823156	-	243	WP_001086388.1	hypothetical protein	-
DTO54_RS25250	4823375..4823593	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
DTO54_RS25260	4824198..4824488	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
DTO54_RS25265	4824489..4824800	-	312	WP_000897305.1	hypothetical protein	Toxin
DTO54_RS25270	4825029..4825937	+	909	WP_001162704.1	alpha/beta hydrolase	-
DTO54_RS25275	4826001..4826942	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
DTO54_RS25280	4826987..4827424	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
DTO54_RS25285	4827421..4828293	-	873	WP_000920762.1	virulence factor BrkB family protein	-
DTO54_RS25290	4828287..4828886	-	600	WP_001295269.1	glucose-1-phosphatase	-
DTO54_RS25295	4828985..4829770	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T108267 WP_000897305.1 NZ_CP030939:c4824800-4824489 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T108267 NZ_CP030939:c4824800-4824489 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT108267 WP_000356397.1 NZ_CP030939:c4824488-4824198 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT108267 NZ_CP030939:c4824488-4824198 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7