Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3257349..3257851 Replicon chromosome
Accession NZ_CP030939
Organism Escherichia coli strain AMSHJX01

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag DTO54_RS17165 Protein ID WP_000767829.1
Coordinates 3257349..3257603 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DTO54_RS17170 Protein ID WP_001259255.1
Coordinates 3257600..3257851 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DTO54_RS17135 3252364..3252591 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
DTO54_RS17140 3252605..3253663 - 1059 WP_000492344.1 transport protein YeeE -
DTO54_RS17145 3253842..3255200 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
DTO54_RS17150 3255190..3255252 - 63 WP_010723108.1 membrane protein YoeI -
DTO54_RS17155 3255467..3256396 - 930 WP_000803351.1 LysR family transcriptional regulator -
DTO54_RS17160 3256442..3257266 - 825 WP_000754749.1 SDR family oxidoreductase -
DTO54_RS17165 3257349..3257603 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DTO54_RS17170 3257600..3257851 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DTO54_RS17175 3258134..3258184 + 51 WP_001364200.1 his operon leader peptide -
DTO54_RS17185 3258330..3259229 + 900 WP_000131777.1 ATP phosphoribosyltransferase -
DTO54_RS17190 3259235..3260539 + 1305 WP_001614652.1 histidinol dehydrogenase -
DTO54_RS17195 3260536..3261606 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
DTO54_RS17200 3261606..3262673 + 1068 WP_001567686.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T108260 WP_000767829.1 NZ_CP030939:c3257603-3257349 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T108260 NZ_CP030939:c3257603-3257349 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT108260 WP_001259255.1 NZ_CP030939:c3257851-3257600 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT108260 NZ_CP030939:c3257851-3257600 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References