Detailed information of TA system TA108201

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3772..4633
• Replicon: plasmid pKL53-S
• Accession: NZ_CP030922
• Organism: Escherichia coli strain KL53

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: CPT07_RS27260
• Protein ID: WP_001548787.1
• Coordinates: 3772..4266 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: CPT07_RS27265
• Protein ID: WP_001548786.1
• Coordinates: 4289..4633 (-)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPT07_RS27245	954..1283	-	330	WP_097360130.1	hypothetical protein	-
CPT07_RS27250	1448..2206	-	759	WP_001548790.1	hypothetical protein	-
CPT07_RS27255	2369..3217	-	849	WP_001548788.1	DUF4942 domain-containing protein	-
CPT07_RS27260	3772..4266	-	495	WP_001548787.1	type II toxin-antitoxin system YhaV family toxin	Toxin
CPT07_RS27265	4289..4633	-	345	WP_001548786.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
CPT07_RS27275	5102..5332	-	231	WP_001548785.1	hypothetical protein	-
CPT07_RS27280	5323..5586	-	264	WP_001548784.1	DUF1380 family protein	-
CPT07_RS27285	5691..5972	-	282	WP_001548783.1	hypothetical protein	-
CPT07_RS27290	6247..6579	-	333	WP_001548780.1	Arc family DNA-binding protein	-
CPT07_RS27300	6938..7174	-	237	WP_024194800.1	hypothetical protein	-
CPT07_RS27305	7273..7827	-	555	WP_001548888.1	hypothetical protein	-
CPT07_RS27310	7903..8400	-	498	WP_001548887.1	DUF1419 domain-containing protein	-
CPT07_RS27315	8582..9019	-	438	WP_001548886.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..77405	77405

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (11-147)

Antitoxin

• PrlF antitoxin (16-114)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 19162.98 Da
• Isoelectric Point: 7.8608

>T108201 WP_001548787.1 NZ_CP030922:c4266-3772 [Escherichia coli]
MEYVVLNGWKIYFHSCFLKQIVELVAKVEALKEAKPNEYLRKKETKLLKAIERVIEERISTDPFNPQYRQGDTLGEENKH
WFRAKFLQQFRLFYRCSDEHKTIIIGWVNDFDTLRAYGSKTDAYKVFSGMLKAGEPPEDWDTLLAQAMKATSTTSVPGFL
DNQI

Nucleotide

• Length: 495 bp

>T108201 NZ_CP030922:c4266-3772 [Escherichia coli]
ATGGAATATGTGGTGTTGAATGGGTGGAAGATTTACTTTCATTCATGCTTTTTGAAGCAGATTGTAGAGTTAGTGGCGAA
GGTTGAGGCTTTAAAAGAAGCAAAGCCGAATGAATACCTGCGCAAGAAGGAAACCAAGCTCCTGAAAGCGATTGAGCGGG
TGATCGAGGAACGGATATCTACCGATCCCTTCAATCCGCAATACCGTCAGGGAGATACTTTAGGCGAGGAAAATAAACAC
TGGTTCCGCGCCAAGTTTCTGCAACAGTTCCGTTTGTTCTATCGTTGCAGTGATGAACACAAAACAATCATTATCGGTTG
GGTTAACGATTTCGATACCCTTCGCGCTTACGGCTCTAAAACTGATGCCTACAAGGTGTTTTCAGGGATGCTAAAGGCAG
GAGAGCCGCCTGAAGACTGGGATACACTTCTCGCTCAGGCTATGAAAGCCACTTCCACAACCTCTGTTCCTGGTTTCCTC
GATAATCAAATCTAG

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 12762.38 Da
• Isoelectric Point: 4.3217

>AT108201 WP_001548786.1 NZ_CP030922:c4633-4289 [Escherichia coli]
MATQYLNSADVSLRAESRLTERSQTTIPAAIRDALHLKPGEYIKYTLLAGGKVIMSRQEEEQDDPVISQFLTFLENDMKN
NPQNIQPVPVTFWERAKALTAGVEVDLDAPLTDD

Nucleotide

• Length: 345 bp

>AT108201 NZ_CP030922:c4633-4289 [Escherichia coli]
ATGGCTACTCAATATTTAAACAGTGCGGACGTGTCACTGCGTGCTGAGTCCCGACTCACTGAACGCAGCCAGACGACTAT
CCCGGCAGCAATACGCGATGCTTTGCACCTCAAGCCAGGGGAGTACATTAAGTACACTTTGCTTGCAGGCGGTAAAGTCA
TCATGTCCCGACAGGAAGAAGAACAGGACGACCCGGTTATATCGCAGTTTCTGACTTTTCTCGAAAACGACATGAAGAAC
AATCCACAGAACATTCAGCCCGTACCGGTGACGTTCTGGGAACGCGCTAAAGCATTAACTGCTGGAGTTGAGGTCGATTT
AGACGCGCCGCTTACCGATGACTAA