Detailed information of TA system TA108197

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 64512..64776
• Replicon: plasmid pKL53-M
• Accession: NZ_CP030921
• Organism: Escherichia coli strain KL53

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: CPT07_RS26985
• Protein ID: WP_001331364.1
• Coordinates: 64624..64776 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	64512..64569 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPT07_RS26970	60722..61792	-	1071	WP_063097584.1	IncI1-type conjugal transfer protein TrbB	-
CPT07_RS26975	61811..63019	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	64512..64569	-	58	NuclAT_0	-	Antitoxin
-	64512..64569	-	58	NuclAT_0	-	Antitoxin
-	64512..64569	-	58	NuclAT_0	-	Antitoxin
-	64512..64569	-	58	NuclAT_0	-	Antitoxin
CPT07_RS26985	64624..64776	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
CPT07_RS26990	64848..65099	-	252	WP_001291964.1	hypothetical protein	-
CPT07_RS28120	65758..65934	-	177	WP_001054904.1	hypothetical protein	-
CPT07_RS26995	66326..66535	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
CPT07_RS27000	66607..67257	-	651	WP_001178507.1	plasmid IncI1-type surface exclusion protein ExcA	-
CPT07_RS27005	67331..69499	-	2169	WP_000698358.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(6)-Id / aph(3'')-Ib / tet(B)	-	1..109118	109118

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T108197 WP_001331364.1 NZ_CP030921:64624-64776 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T108197 NZ_CP030921:64624-64776 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 58 bp

>AT108197 NZ_CP030921:c64569-64512 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin