Detailed information of TA system TA108194

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4793081..4793775
• Replicon: chromosome
• Accession: NZ_CP030919
• Organism: Escherichia coli strain KL53

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: CPT07_RS25085
• Protein ID: WP_001263493.1
• Coordinates: 4793377..4793775 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: CPT07_RS25080
• Protein ID: WP_000554757.1
• Coordinates: 4793081..4793374 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPT07_RS25055	4788898..4789365	+	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-
CPT07_RS25060	4789385..4790101	+	717	WP_000938723.1	FliA/WhiG family RNA polymerase sigma factor	-
CPT07_RS25065	4790114..4790977	+	864	WP_001169518.1	flagellar motor stator protein MotA	-
CPT07_RS25070	4790980..4791903	+	924	WP_001232547.1	putative lateral flagellar export/assembly protein LafU	-
CPT07_RS25075	4791974..4793029	+	1056	WP_001226164.1	DNA polymerase IV	-
CPT07_RS25080	4793081..4793374	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CPT07_RS25085	4793377..4793775	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
CPT07_RS25090	4793785..4794237	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
CPT07_RS25095	4794483..4794686	+	204	Protein_4557	RNA ligase RtcB family protein	-
CPT07_RS25100	4794685..4795206	+	522	Protein_4558	peptide chain release factor H	-
CPT07_RS25105	4795263..4796720	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
CPT07_RS25110	4796981..4797439	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
CPT07_RS25115	4797531..4798775	+	1245	WP_044864403.1	esterase FrsA	-
-	4798035..4798115	+	81	NuclAT_11	-	-
-	4798035..4798115	+	81	NuclAT_11	-	-
-	4798035..4798115	+	81	NuclAT_11	-	-
-	4798035..4798115	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T108194 WP_001263493.1 NZ_CP030919:4793377-4793775 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T108194 NZ_CP030919:4793377-4793775 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT108194 WP_000554757.1 NZ_CP030919:4793081-4793374 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT108194 NZ_CP030919:4793081-4793374 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6