Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1956550..1957052 Replicon chromosome
Accession NZ_CP030919
Organism Escherichia coli strain KL53

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CPT07_RS10585 Protein ID WP_000767829.1
Coordinates 1956550..1956804 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CPT07_RS10590 Protein ID WP_001259255.1
Coordinates 1956801..1957052 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CPT07_RS10555 1951565..1951792 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
CPT07_RS10560 1951806..1952864 - 1059 WP_000492320.1 transport protein YeeE -
CPT07_RS10565 1953043..1954401 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CPT07_RS10570 1954391..1954453 - 63 WP_010723108.1 membrane protein YoeI -
CPT07_RS10575 1954668..1955597 - 930 WP_000803380.1 LysR family transcriptional regulator -
CPT07_RS10580 1955643..1956467 - 825 WP_000754737.1 SDR family oxidoreductase -
CPT07_RS10585 1956550..1956804 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CPT07_RS10590 1956801..1957052 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CPT07_RS10595 1957335..1957385 + 51 WP_001368802.1 his operon leader peptide -
CPT07_RS10605 1957531..1958430 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
CPT07_RS10610 1958436..1959740 + 1305 WP_016239568.1 histidinol dehydrogenase -
CPT07_RS10615 1959737..1960807 + 1071 WP_000108952.1 histidinol-phosphate transaminase -
CPT07_RS10620 1960807..1961874 + 1068 WP_001513846.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T108188 WP_000767829.1 NZ_CP030919:c1956804-1956550 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T108188 NZ_CP030919:c1956804-1956550 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT108188 WP_001259255.1 NZ_CP030919:c1957052-1956801 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT108188 NZ_CP030919:c1957052-1956801 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References