Detailed information of TA system TA108170

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4927126..4927807
• Replicon: chromosome
• Accession: NZ_CP030913
• Organism: Pseudomonas aeruginosa strain Y89

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: Y89_RS23540
• Protein ID: WP_015503432.1
• Coordinates: 4927442..4927807 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: Y89_RS23535
• Protein ID: WP_016561576.1
• Coordinates: 4927126..4927449 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y89_RS23505	4922746..4923624	+	879	WP_003109435.1	hypothetical protein	-
Y89_RS33505	4924136..4925002	-	867	WP_023081971.1	hypothetical protein	-
Y89_RS33510	4925032..4925283	-	252	WP_124136459.1	hypothetical protein	-
Y89_RS23515	4925500..4926054	-	555	WP_074197746.1	tyrosine-type recombinase/integrase	-
Y89_RS23520	4926229..4926504	-	276	WP_004353146.1	hypothetical protein	-
Y89_RS23525	4926504..4926836	-	333	WP_016561578.1	hypothetical protein	-
Y89_RS23530	4926891..4927010	+	120	Protein_4555	IS5/IS1182 family transposase	-
Y89_RS23535	4927126..4927449	-	324	WP_016561576.1	XRE family transcriptional regulator	Antitoxin
Y89_RS23540	4927442..4927807	-	366	WP_015503432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Y89_RS23545	4928071..4928313	-	243	WP_043884955.1	hypothetical protein	-
Y89_RS23550	4928520..4928792	+	273	WP_003085667.1	hypothetical protein	-
Y89_RS23555	4928811..4929236	-	426	WP_003163196.1	VOC family protein	-
Y89_RS23560	4929337..4930221	+	885	WP_019725847.1	LysR family transcriptional regulator	-
Y89_RS23565	4930194..4931147	-	954	WP_003085661.1	LysR family transcriptional regulator	-
Y89_RS23570	4931368..4931802	+	435	WP_003158601.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13798.14 Da
• Isoelectric Point: 4.8219

>T108170 WP_015503432.1 NZ_CP030913:c4927807-4927442 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T108170 NZ_CP030913:c4927807-4927442 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCCTG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCCGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCCATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11903.72 Da
• Isoelectric Point: 5.8900

>AT108170 WP_016561576.1 NZ_CP030913:c4927449-4927126 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQSRGLSQKMLSEVLQVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT108170 NZ_CP030913:c4927449-4927126 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGTCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCAGGTGC
AGCAGCCGGCAATTGCCAAGATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAGCTGGAAGTGATTGCACGCTTCCCGGATGGTGCGGTGAAAATCAGCAACTTCGCGGATATCGAAGAGGCGGGCGC
CTAA