Detailed information of TA system TA108163

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 6054287..6054882
• Replicon: chromosome
• Accession: NZ_CP030912
• Organism: Pseudomonas aeruginosa strain Y82

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: Y82_RS29520
• Protein ID: WP_003117425.1
• Coordinates: 6054604..6054882 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: Y82_RS29515
• Protein ID: WP_003099268.1
• Coordinates: 6054287..6054592 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y82_RS29480	6050930..6051205	-	276	WP_033896065.1	mercury resistance system periplasmic binding protein MerP	-
Y82_RS29485	6051221..6051571	-	351	WP_001294660.1	mercuric transport protein MerT	-
Y82_RS29490	6051643..6052098	+	456	WP_033896066.1	Hg(II)-responsive transcriptional regulator	-
Y82_RS29495	6052460..6053236	-	777	WP_033896079.1	hypothetical protein	-
Y82_RS29500	6053261..6053374	-	114	Protein_5711	RepA	-
Y82_RS29505	6053413..6053625	-	213	WP_025297679.1	AlpA family phage regulatory protein	-
Y82_RS29515	6054287..6054592	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
Y82_RS29520	6054604..6054882	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Y82_RS29530	6055207..6057435	+	2229	WP_003113525.1	TonB-dependent receptor	-
Y82_RS29535	6057505..6058152	-	648	WP_003095021.1	carbonate dehydratase	-
Y82_RS29540	6058214..6059452	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T108163 WP_003117425.1 NZ_CP030912:c6054882-6054604 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T108163 NZ_CP030912:c6054882-6054604 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT108163 WP_003099268.1 NZ_CP030912:c6054592-6054287 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT108163 NZ_CP030912:c6054592-6054287 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5