Detailed information of TA system TA108154

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5796419..5797014
• Replicon: chromosome
• Accession: NZ_CP030911
• Organism: Pseudomonas aeruginosa strain Y71

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: Ysp71_RS27985
• Protein ID: WP_003117425.1
• Coordinates: 5796736..5797014 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: Ysp71_RS27980
• Protein ID: WP_003113527.1
• Coordinates: 5796419..5796724 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Ysp71_RS27945	5791559..5792407	+	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
Ysp71_RS27955	5792574..5793515	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
Ysp71_RS27960	5793632..5794246	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
Ysp71_RS27965	5794288..5794872	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
Ysp71_RS27970	5794913..5796013	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
Ysp71_RS27980	5796419..5796724	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
Ysp71_RS27985	5796736..5797014	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Ysp71_RS27995	5797343..5799571	+	2229	WP_004352671.1	TonB-dependent receptor	-
Ysp71_RS28000	5799641..5800288	-	648	WP_003095021.1	carbonate dehydratase	-
Ysp71_RS28005	5800350..5801588	-	1239	WP_003161380.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T108154 WP_003117425.1 NZ_CP030911:c5797014-5796736 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T108154 NZ_CP030911:c5797014-5796736 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT108154 WP_003113527.1 NZ_CP030911:c5796724-5796419 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT108154 NZ_CP030911:c5796724-5796419 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCTGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5