Detailed information of TA system TA108152

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4990210..4990891
• Replicon: chromosome
• Accession: NZ_CP030911
• Organism: Pseudomonas aeruginosa strain Y71

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: Ysp71_RS24035
• Protein ID: WP_015503432.1
• Coordinates: 4990526..4990891 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: Ysp71_RS24030
• Protein ID: WP_016561576.1
• Coordinates: 4990210..4990533 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Ysp71_RS24000	4985830..4986708	+	879	WP_003109435.1	hypothetical protein	-
Ysp71_RS33705	4987220..4988086	-	867	WP_023081971.1	hypothetical protein	-
Ysp71_RS33710	4988116..4988367	-	252	WP_124136459.1	hypothetical protein	-
Ysp71_RS24010	4988584..4989138	-	555	WP_074197746.1	tyrosine-type recombinase/integrase	-
Ysp71_RS24015	4989313..4989588	-	276	WP_004353146.1	hypothetical protein	-
Ysp71_RS24020	4989588..4989920	-	333	WP_016561578.1	hypothetical protein	-
Ysp71_RS24025	4989975..4990094	+	120	Protein_4639	IS5/IS1182 family transposase	-
Ysp71_RS24030	4990210..4990533	-	324	WP_016561576.1	XRE family transcriptional regulator	Antitoxin
Ysp71_RS24035	4990526..4990891	-	366	WP_015503432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Ysp71_RS24040	4991155..4991397	-	243	WP_043884955.1	hypothetical protein	-
Ysp71_RS24045	4991604..4991876	+	273	WP_003085667.1	hypothetical protein	-
Ysp71_RS24050	4991895..4992320	-	426	WP_003163196.1	VOC family protein	-
Ysp71_RS24055	4992421..4993305	+	885	WP_019725847.1	LysR family transcriptional regulator	-
Ysp71_RS24060	4993278..4994231	-	954	WP_003085661.1	LysR family transcriptional regulator	-
Ysp71_RS24065	4994452..4994886	+	435	WP_003158601.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13798.14 Da
• Isoelectric Point: 4.8219

>T108152 WP_015503432.1 NZ_CP030911:c4990891-4990526 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T108152 NZ_CP030911:c4990891-4990526 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCCTG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCCGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCCATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11903.72 Da
• Isoelectric Point: 5.8900

>AT108152 WP_016561576.1 NZ_CP030911:c4990533-4990210 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQSRGLSQKMLSEVLQVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT108152 NZ_CP030911:c4990533-4990210 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGTCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCAGGTGC
AGCAGCCGGCAATTGCCAAGATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAGCTGGAAGTGATTGCACGCTTCCCGGATGGTGCGGTGAAAATCAGCAACTTCGCGGATATCGAAGAGGCGGGCGC
CTAA