Detailed information of TA system TA108099

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 3618499..3619094
• Replicon: chromosome
• Accession: NZ_CP030861
• Organism: Pseudomonas aeruginosa strain HS9

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: CWE28_RS17485
• Protein ID: WP_003117425.1
• Coordinates: 3618499..3618777 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CWE28_RS17490
• Protein ID: WP_003113527.1
• Coordinates: 3618789..3619094 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWE28_RS17465	3613925..3615163	+	1239	WP_003113524.1	dipeptidase	-
CWE28_RS17470	3615225..3615872	+	648	WP_003095021.1	carbonate dehydratase	-
CWE28_RS17475	3615942..3618170	-	2229	WP_003113525.1	TonB-dependent receptor	-
CWE28_RS17485	3618499..3618777	+	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CWE28_RS17490	3618789..3619094	+	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
CWE28_RS17500	3619501..3620601	-	1101	WP_003099270.1	redox-regulated ATPase YchF	-
CWE28_RS17505	3620642..3621226	-	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
CWE28_RS17510	3621268..3621882	-	615	WP_003095013.1	50S ribosomal protein L25/general stress protein Ctc	-
CWE28_RS17515	3621999..3622940	-	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
CWE28_RS17525	3623107..3623955	-	849	WP_023114865.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T108099 WP_003117425.1 NZ_CP030861:3618499-3618777 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T108099 NZ_CP030861:3618499-3618777 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT108099 WP_003113527.1 NZ_CP030861:3618789-3619094 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT108099 NZ_CP030861:3618789-3619094 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5