Detailed information of TA system TA108067

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 149332..149757
• Replicon: plasmid pAPEC-O2-211A-ColV
• Accession: NZ_CP030791
• Organism: Escherichia coli APEC O2-211

Toxin (Protein)

• Gene name: hok
• Uniprot ID: A0A0D8W959
• Locus tag: APECO2_RS27490
• Protein ID: WP_001332444.1
• Coordinates: 149332..149493 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	149546..149757 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APECO2_RS27455	144682..144909	-	228	WP_001254385.1	conjugal transfer relaxosome protein TraY	-
APECO2_RS27460	145003..145689	-	687	WP_000332490.1	PAS domain-containing protein	-
APECO2_RS27465	145879..146262	-	384	WP_000124981.1	relaxosome protein TraM	-
APECO2_RS27475	146596..147186	+	591	WP_000252676.1	transglycosylase SLT domain-containing protein	-
APECO2_RS27480	147482..148303	-	822	WP_157917628.1	DUF945 domain-containing protein	-
APECO2_RS27485	148482..149094	+	613	Protein_151	hypothetical protein	-
APECO2_RS27490	149332..149493	-	162	WP_001332444.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	149546..149757	-	212	NuclAT_0	-	Antitoxin
-	149546..149757	-	212	NuclAT_0	-	Antitoxin
-	149546..149757	-	212	NuclAT_0	-	Antitoxin
-	149546..149757	-	212	NuclAT_0	-	Antitoxin
APECO2_RS27500	149768..150487	-	720	WP_001276275.1	plasmid SOS inhibition protein A	-
APECO2_RS27505	150484..150918	-	435	WP_000845935.1	conjugation system SOS inhibitor PsiB	-
APECO2_RS27510	150973..151554	-	582	Protein_155	hypothetical protein	-
APECO2_RS27515	151570..152778	-	1209	WP_001352368.1	IS4-like element ISVsa5 family transposase	-
APECO2_RS27525	152887..154269	-	1383	Protein_157	ParB/RepB/Spo0J family partition protein	-
APECO2_RS27530	154328..154561	-	234	WP_001209608.1	DUF905 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / vat / iutA / iucD / iucC / iucB / iucA	1..197773	197773

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5976.19 Da
• Isoelectric Point: 8.5101

>T108067 WP_001332444.1 NZ_CP030791:c149493-149332 [Escherichia coli APEC O2-211]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 162 bp

>T108067 NZ_CP030791:c149493-149332 [Escherichia coli APEC O2-211]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTAATATTCACACTCCTGACCCG
GAATCGTCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAGGTTACGGCAAGTTTGGCTTACGAATCCAGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 212 bp

>AT108067 NZ_CP030791:c149757-149546 [Escherichia coli APEC O2-211]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTCTTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Toxin

• AlphaFold DB: A0A0D8W959

Antitoxin