Detailed information of TA system TA108064

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 111540..111794
• Replicon: plasmid pAPEC-O2-211A-ColV
• Accession: NZ_CP030791
• Organism: Escherichia coli APEC O2-211

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: APECO2_RS27260
• Protein ID: WP_001351576.1
• Coordinates: 111540..111746 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	111733..111794 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APECO2_RS27215	107083..107430	+	348	Protein_102	IS1-like element IS1A family transposase	-
APECO2_RS27225	107870..108157	-	288	WP_000222760.1	type II toxin-antitoxin system RelE/ParE family toxin	-
APECO2_RS27230	108154..108405	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
APECO2_RS27235	109368..110225	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
APECO2_RS27240	110218..110700	-	483	WP_001273588.1	hypothetical protein	-
APECO2_RS27245	110693..110767	-	75	WP_001442103.1	RepA leader peptide Tap	-
APECO2_RS27255	110999..111256	-	258	WP_000083845.1	replication regulatory protein RepA	-
APECO2_RS27260	111540..111746	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	111733..111794	+	62	NuclAT_1	-	Antitoxin
-	111733..111794	+	62	NuclAT_1	-	Antitoxin
-	111733..111794	+	62	NuclAT_1	-	Antitoxin
-	111733..111794	+	62	NuclAT_1	-	Antitoxin
APECO2_RS28155	112050..112124	-	75	Protein_110	endonuclease	-
APECO2_RS27270	112370..112582	-	213	WP_001299730.1	ANR family transcriptional regulator	-
APECO2_RS27275	112718..113278	-	561	WP_000139315.1	fertility inhibition protein FinO	-
APECO2_RS27280	113381..114241	-	861	WP_000704513.1	alpha/beta hydrolase	-
APECO2_RS27285	114300..115046	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / vat / iutA / iucD / iucC / iucB / iucA	1..197773	197773

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T108064 WP_001351576.1 NZ_CP030791:c111746-111540 [Escherichia coli APEC O2-211]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T108064 NZ_CP030791:c111746-111540 [Escherichia coli APEC O2-211]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT108064 NZ_CP030791:111733-111794 [Escherichia coli APEC O2-211]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin