Detailed information of TA system TA108061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 4341249..4341961
• Replicon: chromosome
• Accession: NZ_CP030787
• Organism: Escherichia albertii strain 2013C-4143

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7U8WCQ7
• Locus tag: CX698_RS21960
• Protein ID: WP_000162413.1
• Coordinates: 4341249..4341551 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CX698_RS21965
• Protein ID: WP_000806446.1
• Coordinates: 4341623..4341961 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CX698_RS21940	4337905..4338747	+	843	WP_010326142.1	23S rRNA (adenine(2030)-N(6))-methyltransferase	-
CX698_RS21945	4338819..4340171	+	1353	WP_000160761.1	glutathione-disulfide reductase	-
CX698_RS21950	4340225..4340308	-	84	WP_001295215.1	hypothetical protein	-
CX698_RS23655	4340336..4340509	+	174	WP_000553433.1	hypothetical protein	-
CX698_RS21955	4340599..4341078	-	480	WP_000532312.1	hypothetical protein	-
CX698_RS21960	4341249..4341551	+	303	WP_000162413.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CX698_RS21965	4341623..4341961	+	339	WP_000806446.1	HigA family addiction module antidote protein	Antitoxin
CX698_RS21970	4342018..4342201	+	184	Protein_4166	hypothetical protein	-
CX698_RS21975	4342548..4343114	+	567	WP_001044206.1	outer membrane lipoprotein Slp	-
CX698_RS21980	4343261..4343791	+	531	WP_000480294.1	LuxR family transcriptional regulator	-
CX698_RS21985	4343863..4344891	-	1029	WP_113640809.1	hematinate-forming heme oxygenase ChuS	-
CX698_RS21990	4344940..4346922	-	1983	WP_113640810.1	TonB-dependent heme/hemoglobin receptor ChuA/ShuA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-100)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11871.59 Da
• Isoelectric Point: 9.8664

>T108061 WP_000162413.1 NZ_CP030787:4341249-4341551 [Escherichia albertii]
MTKKINIKDFRDAWLDDFFEFSTPHKKIPSDIHITLLRKLDIINAATTWKDLRSPPGNRYEELSGKLNGYSSIRVNNQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T108061 NZ_CP030787:4341249-4341551 [Escherichia albertii]
ATGACCAAAAAAATTAACATAAAGGATTTCAGGGATGCGTGGCTTGATGATTTTTTTGAATTTTCAACACCACACAAAAA
GATACCTTCTGATATTCATATTACATTGTTGCGGAAGCTGGATATTATCAATGCCGCAACTACCTGGAAGGACTTGAGAT
CACCACCAGGTAATCGGTATGAGGAATTGTCAGGGAAGCTAAACGGCTATTCATCAATAAGGGTGAATAATCAATATAGG
TTAATTTTTAAGTGGGTTAACGGAAAGGCCGAGGACTTGTATCTCGACCCTCACAAATACTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13037.92 Da
• Isoelectric Point: 5.2075

>AT108061 WP_000806446.1 NZ_CP030787:4341623-4341961 [Escherichia albertii]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRITTAEEFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT108061 NZ_CP030787:4341623-4341961 [Escherichia albertii]
ATGAAACAGGCAACCAGAAAACCGACGACTGTAGGTGATATCCTGCTGTACGAATACCTGGAGCCGTTAGAGCTTAAGAT
CAACGAGTTAGCAGAAATACTTCATGTTCATCGCAACACCGTAAGTGCTCTTGTTAATAATAATCGTAAGCTAACGATGG
ATATGGCATATCGCCTGGCAAAAGCATTCGATACTTCTGTAGATTTTTGGATTAATCTTCAGACCGCAGTAGACCTGTGG
GAAGTTGAAAATGATATGCGCGTTCAGGAAGAGTTAAGCCGTATCACTACTGCTGAAGAATTTATTTCTCAGCGGAATCT
GAATAAAAAAGCAGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7U8WCQ7