Detailed information of TA system TA108058

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3512507..3513234
• Replicon: chromosome
• Accession: NZ_CP030787
• Organism: Escherichia albertii strain 2013C-4143

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: CX698_RS17720
• Protein ID: WP_000547563.1
• Coordinates: 3512923..3513234 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CX698_RS17715
• Protein ID: WP_000126297.1
• Coordinates: 3512507..3512926 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CX698_RS17700	3508632..3510884	-	2253	WP_113640904.1	carbamoyltransferase HypF	-
CX698_RS17705	3511037..3511564	-	528	WP_001078777.1	electron transport protein HydN	-
CX698_RS17710	3511987..3512415	+	429	WP_000536065.1	DUF4259 domain-containing protein	-
CX698_RS17715	3512507..3512926	-	420	WP_000126297.1	helix-turn-helix domain-containing protein	Antitoxin
CX698_RS17720	3512923..3513234	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
CX698_RS17725	3513396..3514151	-	756	WP_113640623.1	hypothetical protein	-
CX698_RS17730	3514194..3514664	-	471	WP_000132970.1	hydrogenase maturation peptidase HycI	-
CX698_RS17735	3514657..3515067	-	411	WP_001291928.1	formate hydrogenlyase assembly protein HycH	-
CX698_RS17740	3515064..3515831	-	768	WP_000067397.1	formate hydrogenlyase subunit HycG	-
CX698_RS17745	3515831..3516373	-	543	WP_000493797.1	formate hydrogenlyase subunit HycF	-
CX698_RS17750	3516383..3518092	-	1710	WP_113640624.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T108058 WP_000547563.1 NZ_CP030787:c3513234-3512923 [Escherichia albertii]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T108058 NZ_CP030787:c3513234-3512923 [Escherichia albertii]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15446.45 Da
• Isoelectric Point: 4.4596

>AT108058 WP_000126297.1 NZ_CP030787:c3512926-3512507 [Escherichia albertii]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT108058 NZ_CP030787:c3512926-3512507 [Escherichia albertii]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGACGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCAGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22