Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1685878..1686440 Replicon chromosome
Accession NZ_CP030783
Organism Escherichia albertii strain 2012EL-1823B

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag AYN53_RS08550 Protein ID WP_000605676.1
Coordinates 1686162..1686440 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag AYN53_RS08545 Protein ID WP_000781370.1
Coordinates 1685878..1686162 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYN53_RS08530 1681060..1684107 + 3048 WP_077776584.1 formate dehydrogenase-N subunit alpha -
AYN53_RS08535 1684120..1685004 + 885 WP_059268408.1 formate dehydrogenase N subunit beta -
AYN53_RS08540 1684997..1685650 + 654 WP_000045636.1 formate dehydrogenase-N subunit gamma -
AYN53_RS08545 1685878..1686162 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
AYN53_RS08550 1686162..1686440 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AYN53_RS08555 1686626..1687636 - 1011 WP_113649893.1 alcohol dehydrogenase AdhP -
AYN53_RS08560 1687773..1689470 - 1698 WP_000433442.1 malate dehydrogenase -
AYN53_RS08565 1689627..1689764 - 138 WP_000841563.1 stationary-phase-induced ribosome-associated protein -
AYN53_RS08570 1690022..1690453 + 432 WP_000152289.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T108022 WP_000605676.1 NZ_CP030783:c1686440-1686162 [Escherichia albertii]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T108022 NZ_CP030783:c1686440-1686162 [Escherichia albertii]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCATTAAGCGGCGATCGCGATGGCATCTGGGCAATATCTGTATCAGGCAACTGGCGAATCACGTTTGAGTTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT108022 WP_000781370.1 NZ_CP030783:c1686162-1685878 [Escherichia albertii]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT108022 NZ_CP030783:c1686162-1685878 [Escherichia albertii]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTGAACGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGACTGTTGACCGGAAAAGCGGCTTTGACGCCAGAAATGGCGA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCTGAG
AAAACGGTGGATGTGTCGCGGTTGCGCCGTCTGGTGACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References