Detailed information of TA system TA108018

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1577625..1578031
• Replicon: chromosome
• Accession: NZ_CP030783
• Organism: Escherichia albertii strain 2012EL-1823B

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: -
• Locus tag: AYN53_RS08005
• Protein ID: WP_113650750.1
• Coordinates: 1577801..1578031 (-)
• Length: 77 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1577625..1577802 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYN53_RS25865	1573403..1573576	+	174	WP_032275239.1	hypothetical protein	-
AYN53_RS07980	1573606..1574979	+	1374	WP_059268187.1	ATP-dependent RNA helicase DbpA	-
AYN53_RS07985	1575082..1576017	-	936	WP_113649852.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
AYN53_RS07990	1576069..1577304	-	1236	WP_072249326.1	site-specific integrase	-
AYN53_RS07995	1577306..1577521	-	216	WP_048969784.1	excisionase XisR	-
AYN53_RS08000	1577620..1577808	-	189	WP_059278634.1	DUF1187 family protein	-
-	1577625..1577802	+	178	NuclAT_0	-	Antitoxin
-	1577625..1577802	+	178	NuclAT_0	-	Antitoxin
-	1577625..1577802	+	178	NuclAT_0	-	Antitoxin
-	1577625..1577802	+	178	NuclAT_0	-	Antitoxin
AYN53_RS08005	1577801..1578031	-	231	WP_113650750.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
AYN53_RS08010	1578028..1578708	-	681	WP_113649853.1	YqaJ viral recombinase family protein	-
AYN53_RS08015	1578705..1579484	-	780	WP_000100866.1	phage recombination protein Bet	-
AYN53_RS08020	1579490..1579786	-	297	WP_113649854.1	host-nuclease inhibitor protein Gam	-
AYN53_RS08025	1579764..1581732	-	1969	Protein_1532	exodeoxyribonuclease VIII	-
AYN53_RS08030	1581833..1582108	-	276	WP_113649855.1	hypothetical protein	-
AYN53_RS08035	1582183..1582353	-	171	WP_113649856.1	hypothetical protein	-
AYN53_RS08040	1582353..1582574	-	222	WP_048969779.1	cell division protein FtsZ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	espK / nleD / nleG7'	1576069..1598142	22073

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (18-66)

Sequences

Toxin

Protein

• Length: 77 a.a.
• Molecular weight: 8253.44 Da
• Isoelectric Point: 9.0147

>T108018 WP_113650750.1 NZ_CP030783:c1578031-1577801 [Escherichia albertii]
MSAATKLTGEKPERYTKVKPCPFCGCPSVTVKAISGCYRAKCNGCESRTGYGGSEKEALERWNKRTAENINGGIYV

Nucleotide

• Length: 231 bp

>T108018 NZ_CP030783:c1578031-1577801 [Escherichia albertii]
ATGAGCGCAGCCACAAAGCTCACAGGAGAAAAACCAGAGCGATACACAAAAGTCAAACCATGTCCGTTTTGTGGTTGTCC
ATCTGTAACAGTGAAAGCCATTTCAGGGTGTTACCGGGCAAAATGCAACGGCTGCGAATCCCGAACTGGCTATGGTGGAA
GTGAGAAAGAAGCCCTCGAAAGATGGAATAAACGAACCGCTGAAAATATTAATGGAGGCATCTATGTATAA

Antitoxin

Nucleotide

• Length: 178 bp

>AT108018 NZ_CP030783:1577625-1577802 [Escherichia albertii]
AGGACTGAAGTTTCTCGCAATTAAAATTCATCAGTTTTACATTTTGCTCTCTGGAAACGCCTGCTTCTTTTTTCCCTGAG
AGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTACCTCCTTCCTTTTC
AATTGTGGCGGTAATTTT

Structures

Antitoxin