Detailed information of TA system TA108016

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 386550..387244
• Replicon: chromosome
• Accession: NZ_CP030783
• Organism: Escherichia albertii strain 2012EL-1823B

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: AYN53_RS01895
• Protein ID: WP_001263500.1
• Coordinates: 386846..387244 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: AYN53_RS01890
• Protein ID: WP_000554758.1
• Coordinates: 386550..386843 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYN53_RS01870	382248..382745	+	498	WP_095573642.1	REP-associated tyrosine transposase RayT	-
AYN53_RS01875	382861..384573	-	1713	Protein_357	flagellar biosynthesis protein FlhA	-
AYN53_RS01880	384545..385312	+	768	WP_113650736.1	putative lateral flagellar export/assembly protein LafU	-
AYN53_RS01885	385443..386498	+	1056	WP_113649515.1	DNA polymerase IV	-
AYN53_RS01890	386550..386843	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
AYN53_RS01895	386846..387244	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
AYN53_RS01900	387254..387706	+	453	WP_095575504.1	GNAT family N-acetyltransferase	-
AYN53_RS01905	387719..388318	+	600	WP_072249376.1	peptide chain release factor H	-
AYN53_RS01915	388459..388701	-	243	WP_059222190.1	hypothetical protein	-
AYN53_RS01920	388760..390217	-	1458	WP_125295743.1	cytosol nonspecific dipeptidase	-
AYN53_RS01925	390478..390936	+	459	WP_001291989.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T108016 WP_001263500.1 NZ_CP030783:386846-387244 [Escherichia albertii]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T108016 NZ_CP030783:386846-387244 [Escherichia albertii]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT108016 WP_000554758.1 NZ_CP030783:386550-386843 [Escherichia albertii]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT108016 NZ_CP030783:386550-386843 [Escherichia albertii]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3