Detailed information of TA system TA108006

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 75878..76301
• Replicon: plasmid unnamed
• Accession: NZ_CP030782
• Organism: Escherichia albertii strain 07-3866

Toxin (Protein)

• Gene name: hok
• Uniprot ID: Q2VNZ0
• Locus tag: CCF18_RS24875
• Protein ID: WP_000775238.1
• Coordinates: 76140..76301 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	75878..76094 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCF18_RS24845	71192..72172	-	981	WP_164512216.1	IS110 family transposase	-
CCF18_RS24850	72285..73453	+	1169	WP_125317555.1	IS3 family transposase	-
CCF18_RS24855	73676..74209	+	534	WP_125317917.1	single-stranded DNA-binding protein	-
CCF18_RS24860	74267..74500	+	234	WP_001478623.1	DUF905 family protein	-
CCF18_RS25190	74493..74662	+	170	Protein_81	chromosome partitioning protein ParB	-
CCF18_RS24865	74717..75151	+	435	WP_125317918.1	conjugation system SOS inhibitor PsiB	-
CCF18_RS24870	75148..75866	+	719	Protein_83	plasmid SOS inhibition protein A	-
-	75878..76094	+	217	NuclAT_0	-	Antitoxin
-	75878..76094	+	217	NuclAT_0	-	Antitoxin
-	75878..76094	+	217	NuclAT_0	-	Antitoxin
-	75878..76094	+	217	NuclAT_0	-	Antitoxin
CCF18_RS24875	76140..76301	+	162	WP_000775238.1	type I toxin-antitoxin system Hok family toxin	Toxin
CCF18_RS24885	76923..77122	+	200	Protein_85	hypothetical protein	-
CCF18_RS24890	77241..78062	+	822	Protein_86	DUF945 domain-containing protein	-
CCF18_RS24895	78358..78951	-	594	WP_125317933.1	transglycosylase SLT domain-containing protein	-
CCF18_RS24905	79287..79670	+	384	WP_000124981.1	relaxosome protein TraM	-
CCF18_RS24910	79860..80546	+	687	WP_125317919.1	PAS domain-containing protein	-
CCF18_RS24915	80640..80867	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
CCF18_RS24920	80901..81266	+	366	WP_000340270.1	type IV conjugative transfer system pilin TraA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	faeC / faeD / faeD / faeE / faeF / faeH / faeI / espL2 / espL2 / espL2	1..104269	104269
	-	flank	IS/Tn	-	-	72285..72587	302

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T108006 WP_000775238.1 NZ_CP030782:76140-76301 [Escherichia albertii]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 162 bp

>T108006 NZ_CP030782:76140-76301 [Escherichia albertii]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGTTGCACGTTGTTAATATTCACACTCCTGACCCG
GAATCGCCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAAGTTACGGCAACAATGGCTTACGAATCCGGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 217 bp

>AT108006 NZ_CP030782:75878-76094 [Escherichia albertii]
TCACACAGATTACCTGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTATCCTGTCGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTCATTAACCCACGAGGCCTCTGCATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: A0A7Z7YI12

Antitoxin