Detailed information of TA system TA107994

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 3603301..3604013
• Replicon: chromosome
• Accession: NZ_CP030781
• Organism: Escherichia albertii strain 07-3866

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7U8WCQ7
• Locus tag: CCF18_RS18075
• Protein ID: WP_000162413.1
• Coordinates: 3603301..3603603 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CCF18_RS18080
• Protein ID: WP_000806447.1
• Coordinates: 3603675..3604013 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCF18_RS18055	3599964..3600806	+	843	WP_125317703.1	23S rRNA (adenine(2030)-N(6))-methyltransferase	-
CCF18_RS18060	3600878..3602230	+	1353	WP_059273528.1	glutathione-disulfide reductase	-
CCF18_RS18065	3602284..3602367	-	84	WP_001295215.1	hypothetical protein	-
CCF18_RS25150	3602395..3602568	+	174	WP_059225143.1	hypothetical protein	-
CCF18_RS18070	3602658..3603137	-	480	WP_000532312.1	hypothetical protein	-
CCF18_RS18075	3603301..3603603	+	303	WP_000162413.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CCF18_RS18080	3603675..3604013	+	339	WP_000806447.1	HigA family addiction module antidote protein	Antitoxin
CCF18_RS18085	3604070..3604249	+	180	WP_002460167.1	hypothetical protein	-
CCF18_RS18090	3604596..3605162	+	567	WP_001044206.1	outer membrane lipoprotein Slp	-
CCF18_RS18095	3605309..3605839	+	531	WP_000480294.1	LuxR family transcriptional regulator	-
CCF18_RS18100	3605911..3606939	-	1029	WP_001110408.1	hematinate-forming heme oxygenase ChuS	-
CCF18_RS18105	3606988..3608971	-	1984	Protein_3460	TonB-dependent heme/hemoglobin receptor ChuA/ShuA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11871.59 Da
• Isoelectric Point: 9.8664

>T107994 WP_000162413.1 NZ_CP030781:3603301-3603603 [Escherichia albertii]
MTKKINIKDFRDAWLDDFFEFSTPHKKIPSDIHITLLRKLDIINAATTWKDLRSPPGNRYEELSGKLNGYSSIRVNNQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T107994 NZ_CP030781:3603301-3603603 [Escherichia albertii]
ATGACCAAAAAAATTAACATAAAGGATTTCAGGGATGCGTGGCTTGATGATTTTTTTGAATTTTCAACACCACACAAAAA
GATACCTTCTGATATTCATATTACATTGTTGCGGAAGCTGGATATTATCAATGCCGCAACTACCTGGAAGGACTTGAGAT
CACCACCAGGTAATCGGTATGAGGAATTGTCAGGGAAGCTAAACGGCTATTCATCAATAAGGGTGAATAATCAATATAGG
TTAATTTTTAAGTGGGTTAACGGAAAGGCCGAGGACTTGTATCTCGACCCTCACAAATACTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13010.90 Da
• Isoelectric Point: 5.2075

>AT107994 WP_000806447.1 NZ_CP030781:3603675-3604013 [Escherichia albertii]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNSRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRITTAEEFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT107994 NZ_CP030781:3603675-3604013 [Escherichia albertii]
ATGAAACAGGCAACCAGAAAACCGACGACTGTAGGTGATATCCTGCTGTACGAATACCTGGAGCCGTTAGAGCTTAAGAT
CAACGAGTTAGCAGAAATACTTCATGTTCATCGCAACACCGTAAGTGCTCTTGTTAATAATAGTCGTAAGCTAACGATGG
ATATGGCATATCGCCTGGCAAAAGCATTCGATACTTCTGTAGATTTTTGGATTAATCTTCAGACCGCAGTAGACCTGTGG
GAAGTTGAAAATGATATGCGCGTTCAGGAAGAGTTAAGCCGTATCACTACTGCTGAAGAATTTATTTCTCAGCGGAATCT
GAATAAAAAAGCAGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7U8WCQ7