Detailed information of TA system TA107966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3987855..3988582
• Replicon: chromosome
• Accession: NZ_CP030778
• Organism: Escherichia albertii strain 05-3106

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: CYM74_RS19575
• Protein ID: WP_000547563.1
• Coordinates: 3987855..3988166 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CYM74_RS19580
• Protein ID: WP_000126297.1
• Coordinates: 3988163..3988582 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CYM74_RS19545	3982997..3984706	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
CYM74_RS19550	3984716..3985258	+	543	WP_000493797.1	formate hydrogenlyase subunit HycF	-
CYM74_RS19555	3985258..3986025	+	768	WP_000067396.1	formate hydrogenlyase subunit HycG	-
CYM74_RS19560	3986022..3986432	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
CYM74_RS19565	3986425..3986895	+	471	WP_059219259.1	hydrogenase maturation peptidase HycI	-
CYM74_RS19570	3986938..3987693	+	756	WP_113623175.1	hypothetical protein	-
CYM74_RS19575	3987855..3988166	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
CYM74_RS19580	3988163..3988582	+	420	WP_000126297.1	helix-turn-helix domain-containing protein	Antitoxin
CYM74_RS19585	3988674..3989102	-	429	WP_000536064.1	hypothetical protein	-
CYM74_RS19595	3989531..3990058	+	528	WP_001078780.1	electron transport protein HydN	-
CYM74_RS19600	3990211..3992462	+	2252	Protein_3754	carbamoyltransferase HypF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T107966 WP_000547563.1 NZ_CP030778:3987855-3988166 [Escherichia albertii]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T107966 NZ_CP030778:3987855-3988166 [Escherichia albertii]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15446.45 Da
• Isoelectric Point: 4.4596

>AT107966 WP_000126297.1 NZ_CP030778:3988163-3988582 [Escherichia albertii]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT107966 NZ_CP030778:3988163-3988582 [Escherichia albertii]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGACGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCAGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22