Detailed information of TA system TA107959

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2708728..2709330
• Replicon: chromosome
• Accession: NZ_CP030778
• Organism: Escherichia albertii strain 05-3106

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: CYM74_RS13340
• Protein ID: WP_000897302.1
• Coordinates: 2709019..2709330 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CYM74_RS13335
• Protein ID: WP_000356397.1
• Coordinates: 2708728..2709018 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CYM74_RS13295	2704127..2705029	+	903	WP_113623021.1	formate dehydrogenase O subunit beta	-
CYM74_RS13300	2705026..2705661	+	636	WP_000829019.1	formate dehydrogenase cytochrome b556 subunit	-
CYM74_RS13305	2705658..2706587	+	930	WP_000027704.1	formate dehydrogenase accessory protein FdhE	-
CYM74_RS13310	2706624..2706998	-	375	WP_044713737.1	type II toxin-antitoxin system VapC family toxin	-
CYM74_RS13315	2706998..2707240	-	243	Protein_2559	CopG family transcriptional regulator	-
CYM74_RS13320	2707458..2707676	-	219	WP_001251292.1	ribbon-helix-helix domain-containing protein	-
CYM74_RS13325	2708091..2708369	-	279	WP_044710309.1	hypothetical protein	-
CYM74_RS13330	2708431..2708643	-	213	WP_000197774.1	hypothetical protein	-
CYM74_RS13335	2708728..2709018	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CYM74_RS13340	2709019..2709330	-	312	WP_000897302.1	hypothetical protein	Toxin
CYM74_RS13345	2709559..2710467	+	909	WP_059239594.1	alpha/beta hydrolase	-
CYM74_RS13350	2710531..2711472	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CYM74_RS13355	2711517..2711954	-	438	WP_000560977.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CYM74_RS13360	2711951..2712823	-	873	WP_000916663.1	virulence factor BrkB family protein	-
CYM74_RS13365	2712817..2713416	-	600	WP_002460585.1	glucose-1-phosphatase	-
CYM74_RS13370	2713515..2713955	-	441	Protein_2570	DeoR/GlpR transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T107959 WP_000897302.1 NZ_CP030778:c2709330-2709019 [Escherichia albertii]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T107959 NZ_CP030778:c2709330-2709019 [Escherichia albertii]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT107959 WP_000356397.1 NZ_CP030778:c2709018-2708728 [Escherichia albertii]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT107959 NZ_CP030778:c2709018-2708728 [Escherichia albertii]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416