Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 5110424..5110926 Replicon chromosome
Accession NZ_CP030768
Organism Escherichia coli strain 2017C-4173W12

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag DSB71_RS26910 Protein ID WP_000767829.1
Coordinates 5110424..5110678 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DSB71_RS26915 Protein ID WP_001259255.1
Coordinates 5110675..5110926 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DSB71_RS26880 5105439..5105666 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
DSB71_RS26885 5105680..5106738 - 1059 WP_023910197.1 transport protein YeeE -
DSB71_RS26890 5106917..5108275 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
DSB71_RS26895 5108265..5108327 - 63 WP_010723108.1 membrane protein YoeI -
DSB71_RS26900 5108542..5109471 - 930 WP_000803351.1 LysR family transcriptional regulator -
DSB71_RS26905 5109517..5110341 - 825 WP_000754749.1 SDR family oxidoreductase -
DSB71_RS26910 5110424..5110678 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DSB71_RS26915 5110675..5110926 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DSB71_RS26920 5111209..5111259 + 51 WP_001364200.1 his operon leader peptide -
DSB71_RS26930 5111405..5112304 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
DSB71_RS26935 5112310..5113614 + 1305 WP_001559366.1 histidinol dehydrogenase -
DSB71_RS26940 5113611..5114681 + 1071 WP_000108970.1 histidinol-phosphate transaminase -
DSB71_RS26945 5114681..5115748 + 1068 WP_001563933.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T107927 WP_000767829.1 NZ_CP030768:c5110678-5110424 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T107927 NZ_CP030768:c5110678-5110424 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT107927 WP_001259255.1 NZ_CP030768:c5110926-5110675 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT107927 NZ_CP030768:c5110926-5110675 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References