107927

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 5110424..5110926 Replicon chromosome
Accession NZ_CP030768
Organism Escherichia coli strain 2017C-4173W12

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag DSB71_RS26910 Protein ID WP_000767829.1
Coordinates 5110424..5110678 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DSB71_RS26915 Protein ID WP_001259255.1
Coordinates 5110675..5110926 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DSB71_RS26880 5105439..5105666 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
DSB71_RS26885 5105680..5106738 - 1059 WP_023910197.1 transport protein YeeE -
DSB71_RS26890 5106917..5108275 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
DSB71_RS26895 5108265..5108327 - 63 WP_010723108.1 membrane protein YoeI -
DSB71_RS26900 5108542..5109471 - 930 WP_000803351.1 LysR family transcriptional regulator -
DSB71_RS26905 5109517..5110341 - 825 WP_000754749.1 SDR family oxidoreductase -
DSB71_RS26910 5110424..5110678 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DSB71_RS26915 5110675..5110926 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DSB71_RS26920 5111209..5111259 + 51 WP_001364200.1 his operon leader peptide -
DSB71_RS26930 5111405..5112304 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
DSB71_RS26935 5112310..5113614 + 1305 WP_001559366.1 histidinol dehydrogenase -
DSB71_RS26940 5113611..5114681 + 1071 WP_000108970.1 histidinol-phosphate transaminase -
DSB71_RS26945 5114681..5115748 + 1068 WP_001563933.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T107927 WP_000767829.1 NZ_CP030768:c5110678-5110424 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T107927 NZ_CP030768:c5110678-5110424 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT107927 WP_001259255.1 NZ_CP030768:c5110926-5110675 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT107927 NZ_CP030768:c5110926-5110675 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References